ArticleGenetics2021
A genetic titration of membrane composition in Caenorhabditis elegans reveals its importance for multiple cellular and physiological traits.
Article in Genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- A pcyt-1 allelic series reveals in vivo consequences of reduced phosphatidylcholine synthesis in C. elegans.G3 (Bethesda, Md.) · 2026Article
- Forward genetics identifies cuticle and regulatory genes underlying cellular boundary integrity in C. elegans.G3 (Bethesda, Md.) · 2026Article
- AbioRxiv : the preprint server for biology · 2026Article
- SWI/SNF complexes modulate gene expression and the development of physical dependence to ethanol.Alcohol, clinical & experimental research · 2026Article
- Forward genetics ineLife · 2025Article
- An inducible and reversible system to regulate unsaturated fatty acid biosynthesis in C. elegans.G3 (Bethesda, Md.) · 2025Article
- Lipid Oxidation at the Crossroads: Oxidative Stress and Neurodegeneration Explored inAntioxidants (Basel, Switzerland) · 2025Review
- What's past is prologue: FRAP keeps delivering 50 years later.Biophysical journal · 2023Review
- AdipoR2 recruits protein interactors to promote fatty acid elongation and membrane fluidity.The Journal of biological chemistry · 2023Article
- Diet effects on longevity, heat tolerance, lipid peroxidation and mitochondrial membrane potential in Daphnia.Oecologia · 2023Article
- Sphingosine 1-phosphate mediates adiponectin receptor signaling essential for lipid homeostasis and embryogenesis.Nature communications · 2022Article
- A conserved megaprotein-based molecular bridge critical for lipid trafficking and cold resilience.Nature communications · 2022Article
- Bacterial diet modulates tamoxifen-induced death via host fatty acid metabolism.Nature communications · 2022Article
- Ether lipid deficiency disrupts lipid homeostasis leading to ferroptosis sensitivity.PLoS genetics · 2022Article
- Oxidation of Polyunsaturated Fatty Acids as a Promising Area of Research in Infertility.Antioxidants (Basel, Switzerland) · 2022Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Communicating editor: B. Grant The composition and biophysical properties of cellular membranes must be tightly regulated to maintain the proper functions of myriad processes within cells. To better understand the importance of membrane homeostasis, we assembled a panel of five Caenorhabditis elegans strains that show a wide span of membrane composition and properties, ranging from excessively rich in saturated fatty acids (SFAs) and rigid to excessively rich in polyunsaturated fatty acids (PUFAs) and fluid. The genotypes of the five strain are, from most rigid to most fluid: paqr-1(tm3262); paqr-2(tm3410), paqr-2(tm3410), N2 (wild-type), mdt-15(et14); nhr-49(et8), and mdt-15(et14); nhr-49(et8); acs-13(et54). We confirmed the excess SFA/rigidity-to-excess PUFA/fluidity gradient using the methods of fluorescence recovery after photobleaching (FRAP) and lipidomics analysis. The five strains were then studied for a variety of cellular and physiological traits and found to exhibit defects in: permeability, lipid peroxidation, growth at different temperatures, tolerance to SFA-rich diets, lifespan, brood size, vitellogenin trafficking, oogenesis, and autophagy during starvation. The excessively rigid strains often exhibited defects in opposite directions compared to the excessively fluid strains. We conclude that deviation from wild-type membrane homeostasis is pleiotropically deleterious for numerous cellular/physiological traits. The strains introduced here should prove useful to further study the cellular and physiological consequences of impaired membrane homeostasis.
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