ArticlePloS one2021
Intragenomic sequence variations in the second internal transcribed spacer (ITS2) ribosomal DNA of the malaria vector Anopheles stephensi.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 29 citations in OpenAlex.
- A multiplex assay to detect mosquito species, bloodmeal host source and Plasmodium in malaria vectors using Nanopore amplicon sequencing.Medical and veterinary entomology · 2026Article
- Xenopsylla buxtoni fleas as a dominant species harboring multiple infections of Wolbachia lineages in the ancient plague epicenters of Iran.PLoS neglected tropical diseases · 2026Article
- Epidemiology, morphometry, and phylogenetic analysis ofHelminthologia · 2025Article
- First detection and entomological characterisation of invasive malaria vector Anopheles stephensi in sympatry with other vectors in Gayi Southern Niger Republic.Scientific reports · 2025Article
- Topic: Distribution of Anopheles stephensi bioforms in selected districts of Rajasthan, India.PloS one · 2025Article
- NewStudies in mycology · 2024Article
- Loop-Mediated Isothermal Amplification Assay to Detect Invasive Malaria Vector Anopheles stephensi Mosquitoes.Emerging infectious diseases · 2024Article
- A decade of invasive Anopheles stephensi sequence-based identification: toward a global standard.Trends in parasitology · 2024Review
- Revolutionizing Malaria Vector Control: The Importance of Accurate Species Identification through Enhanced Molecular Capacity.Microorganisms · 2023Review
- Identification of two insecticide resistance markers in Ethiopian Anopheles stephensi mosquitoes using a multiplex amplicon sequencing assay.Scientific reports · 2023Article
- Molecular Tools for Early Detection of Invasive Malaria Vector Anopheles stephensi Mosquitoes.Emerging infectious diseases · 2023Article
- Multiple invasions, Wolbachia and human-aided transport drive the genetic variability of Aedes albopictus in the Iberian Peninsula.Scientific reports · 2022Article
- Tandem duplication of a genomic region encoding glutathione S-transferase epsilon-2 and -4 genes in DDT-resistant Anopheles stephensi strain from India.Scientific reports · 2022Article
- Wolbachia 16S rRNA haplotypes detected in wild Anopheles stephensi in eastern Ethiopia.Parasites & vectors · 2022Article
- Article
- Identification of a biological form in the Anopheles stephensi laboratory colony using the odorant-binding protein 1 intron I sequence.PloS one · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Second Internal Transcribed Spacer (ITS2) ribosomal DNA (rDNA) sequence is a widely used molecular marker for species-identification or -delimitation due to observed concerted evolution which is believed to homogenize rDNA copies in an interbreeding population. However, intra-specific differences in ITS2 of Anopheles stephensi have been reported. This study reports the presence of intragenomic sequence variation in the ITS2-rDNA of An. stephensi and hypothesizes that observed intra-specific differences in this species may have resulted due to ambiguous DNA sequence-chromatogram resulting from intragenomic heterogeneity. Anopheles stephensi collected from different parts of India were sequenced for complete ITS2 and the variable region of 28S-rDNA (d1-d3 domains). Intragenomic variations were found in ITS2 region of all An. stephensi sequenced, but no such variation was observed in d1 to d3 domains of 28S-rDNA. Cloning and sequencing of ITS2 through the d3 domain of the 28S region of rDNA from representative samples from northern, central, and southern India confirmed the presence of intragenomic variation in ITS2 due to transitions at three loci and two bp indel in a di-nucleotide microsatellite locus. Multiple haplotypes were observed in ITS2 raised from such variations. Due to the absence of detectable intragenomic sequence variation in the d1 to d3 domain of 28S rDNA of An. stephensi, this region can serve as an ideal reference sequence for taxonomic and phylogenetic studies. The presence of intragenomic variation in rDNA should be carefully examined before using this as a molecular marker for species delimitation or phylogenetic analyses.
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