ArticleNano letters2021
Correlating Super-Resolution Microscopy and Transmission Electron Microscopy Reveals Multiparametric Heterogeneity in Nanoparticles.
Article in Nano letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Advances in Targeted Engineered Nanoparticle-Based Therapeutics for Respiratory Diseases: Current Insights and Future Perspectives.International journal of nanomedicine · 2025Review
- Nanotherapeutic Heterogeneity: Sources, Effects, and Solutions.Small (Weinheim an der Bergstrasse, Germany) · 2024Review
- Single Particle Chemical Characterisation of Nanoformulations for Cargo Delivery.The AAPS journal · 2023Review
- Article
- Multivalent effect of peptide functionalized polymeric nanoparticles towards selective prostate cancer targeting.Nanoscale advances · 2023Article
- Opportunities in optical and electrical single-cell technologies to study microbial ecosystems.Frontiers in microbiology · 2023Review
- Mapping the relationship between total and functional antibodies conjugated to nanoparticles with spectrally-resolved direct stochastic optical reconstruction microscopy (SR-dSTORM).Nanoscale advances · 2022Article
- Fluorescent Nanoparticles for Super-Resolution Imaging.Chemical reviews · 2022Review
- Hysteresis and Stochastic Fluorescence by Aggregated Ensembles of Graphene Quantum Dots.The journal of physical chemistry. C, Nanomaterials and interfaces · 2022Article
- The Interaction of Hypericin with SARS-CoV-2 Reveals a Multimodal Antiviral Activity.ACS applied materials & interfaces · 2022Article
- A Single-Molecule View at Nanoparticle Targeting Selectivity: Correlating Ligand Functionality and Cell Receptor Density.ACS nano · 2022Article
- Can super-resolution microscopy become a standard characterization technique for materials chemistry?Chemical science · 2022Review
- Recent Developments in Correlative Super-Resolution Fluorescence Microscopy and Electron Microscopy.Molecules and cells · 2022Review
- Quantifying the effect of PEG architecture on nanoparticle ligand availability using DNA-PAINT.Nanoscale advances · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The functionalization of nanoparticles with functional moieties is a key strategy to achieve cell targeting in nanomedicine. The interplay between size and ligand number is crucial for the formulation performance and needs to be properly characterized to understand nanoparticle structure-activity relations. However, there is a lack of methods able to measure both size and ligand number at the same time and at the single particle level. Here, we address this issue by introducing a correlative light and electron microscopy (CLEM) method combining super-resolution microscopy (SRM) and transmission electron microscopy (TEM) imaging. We apply our super-resCLEM method to characterize the relationship between size and ligand number and density in PLGA-PEG nanoparticles. We highlight how heterogeneity found in size can impact ligand distribution and how a significant part of the nanoparticle population goes completely undetected in the single-technique analysis. Super-resCLEM holds great promise for the multiparametric analysis of other parameters and nanomaterials.
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Registered trials
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