ArticleThe Plant cell2021
ESCRT components ISTL1 andLIP5 are required for tapetal function and pollen viability.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Single-Cell Transcriptome Atlas and Dynamic Regulatory Mechanisms of Anther Development in Alfalfa (Medicago sativa L.).Plant biotechnology journal · 2025Article
- Insights into the Molecular Basis of Pollen Coat Development and Its Role in Male Sterility.International journal of molecular sciences · 2025Review
- The green ESCRTs: Newly defined roles for ESCRT proteins in plants.The Journal of biological chemistry · 2025Review
- Endosomal membrane budding patterns in plants.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Integrative multi-omic analysis identifies genes associated with cuticular wax biogenesis in adult maize leaves.G3 (Bethesda, Md.) · 2024Article
- Ascorbic acid-mediated reactive oxygen species homeostasis modulates the switch from tapetal cell division to cell differentiation in Arabidopsis.The Plant cell · 2023Article
- Article
- Review
- AP1/2β-mediated exocytosis of tapetum-specific transporters is required for pollen development in Arabidopsis thaliana.The Plant cell · 2022Article
- Ubiquitination of the ubiquitin-binding machinery: how early ESCRT components are controlled.Essays in biochemistry · 2022Review
- Integrating GWAS and TWAS to elucidate the genetic architecture of maize leaf cuticular conductance.Plant physiology · 2022Article
- Electron microscopy for imaging organelles in plants and algae.Plant physiology · 2022Article
- Fifteen compelling open questions in plant cell biology.The Plant cell · 2022Review
- A glossary of plant cell structures: Current insights and future questions.The Plant cell · 2022Review
- Vacuoles in Bryophytes: Properties, Biogenesis, and Evolution.Frontiers in plant science · 2022Review
- A Subunit of ESCRT-III, MoIst1, Is Involved in Fungal Development, Pathogenicity, and Autophagy inFrontiers in plant science · 2022Article
- Bioimaging tools move plant physiology studies forward.Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pollen wall assembly is crucial for pollen development and plant fertility. The durable biopolymer sporopollenin and the constituents of the tryphine coat are delivered to developing pollen grains by the highly coordinated secretory activity of the surrounding tapetal cells. The role of membrane trafficking in this process, however, is largely unknown. In this study, we used Arabidopsis thaliana to characterize the role of two late-acting endosomal sorting complex required for transport (ESCRT) components, ISTL1 and LIP5, in tapetal function. Plants lacking ISTL1 and LIP5 form pollen with aberrant exine patterns, leading to partial pollen lethality. We found that ISTL1 and LIP5 are required for exocytosis of plasma membrane and secreted proteins in the tapetal cells at the free microspore stage, contributing to pollen wall development and tryphine deposition. Whereas the ESCRT machinery is well known for its role in endosomal trafficking, the function of ISTL1 and LIP5 in exocytosis is not a typical ESCRT function. The istl1 lip5 double mutants also show reduced intralumenal vesicle concatenation in multivesicular endosomes in both tapetal cells and developing pollen grains as well as morphological defects in early endosomes/trans-Golgi networks, suggesting that late ESCRT components function in the early endosomal pathway and exocytosis.
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