ArticleBio-protocol2021
Screening for Lysogen Activity in Therapeutically Relevant Bacteriophages.
Article in Bio-protocol, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 31 citations in OpenAlex.
- A Novel Lytic Podovirus AP-20-A InfectingInternational journal of molecular sciences · 2026Article
- Phenotypic and Genomic Characterization of Novel Straboviridae Bacteriophages Targeting Multidrug-ResistantMicroorganisms · 2026Article
- Smooth-to-rough morphotype switching, a mechanism of phage resistance inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Discovery and characterization of a novelPHAGE (New Rochelle, N.Y.) · 2026Article
- The isolation and characterisation of a temperate phage ɸPh_AJ01 against Acinetobacter junii.Virology journal · 2025Article
- Comparative Analysis Reveals Host Species-Dependent Diversity Among 16 Virulent Bacteriophages Isolated Against SoybeanViruses · 2025Article
- Comparative Analysis Reveals Host Species-Dependent Diversity Among 16 Virulent Bacteriophages Isolated Against SoybeanbioRxiv : the preprint server for biology · 2025Article
- Review
- Genomic and Functional Characterization of Novel Phages Targeting Multidrug-ResistantInternational journal of molecular sciences · 2025Article
- Re-evaluating what makes a phage unsuitable for therapy.npj antimicrobials and resistance · 2025Review
- Sphae: an automated toolkit for predicting phage therapy candidates from sequencing data.Bioinformatics advances · 2025Article
- Bacteriophage Therapy as a Promising Alternative for Antibiotic-ResistantAntibiotics (Basel, Switzerland) · 2024Review
- phage therapy candidates from Sphae: An automated toolkit for predicting sequencing data.bioRxiv : the preprint server for biology · 2024Article
- DeepPL: A deep-learning-based tool for the prediction of bacteriophage lifecycle.PLoS computational biology · 2024Article
- Isolation and Characterization of Six NovelPHAGE (New Rochelle, N.Y.) · 2024Article
- Diversity ofMicrobiology spectrum · 2024Article
- Trade-offs between receptor modification and fitness drive host-bacteriophage co-evolution leading to phage extinction or co-existence.The ISME journal · 2024Article
- Isolation and preliminary characterization of a novel bacteriophage vB_KquU_φKuK6 that infects the multidrug-resistant pathogenFrontiers in microbiology · 2024Article
- Effective Isolation and Characterization of Mycobacteriophages with the Ability to LyseViruses · 2023Article
- Knowing and Naming: Phage Annotation and Nomenclature for Phage Therapy.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lysogenic phages can integrate into their bacterial host's genome, potentially transferring any genetic information they possess including virulence or resistance genes, and are therefore routinely excluded from therapeutic applications. Lysogenic behavior is typically seen in phages that create turbid plaques or possess subpar bactericidal activity; yet, these are not definitive indicators. As a result, the presence of integrase genes is often used as a hallmark for lysogenic behavior; however, the accuracy of genetic screening for lysogeny depends on the quality of the extraction, sequencing and assembly of the phage genome, and database comparison. The present protocol describes a simple phenotypic test that can be used to screen therapeutically relevant phages for lysogenic behavior. This test relies on the identification of spontaneous phage release from their lysogenized host and can be reliably used in cases where no sequencing data are available. The protocol does not require specialized equipment, is not work-intensive, and is broadly applicable to any phage with an easily culturable bacterial host, making it particularly amenable to settings with limited resources. Graphical abstract: Screening pipeline for lysogen activity of a given phage.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.