ArticleFrontiers in cell and developmental biology2021
Metabolite Profiling of Pig Seminal Plasma Identifies Potential Biomarkers for Sperm Resilience to Liquid Preservation.
Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 25 citations in OpenAlex.
- Optimization of Semen Extender for Mongolian Sheep by Integrating Metabolomics and Sperm Quality Analysis at 17 °C with Antioxidant Supplementation.Animals : an open access journal from MDPI · 2026Article
- Modest contribution of metabolomic data to genomic prediction of breeding values for feed conversion ratio in pigs.Genetics, selection, evolution : GSE · 2026Article
- Succinylation regulates boar sperm linear motility via reprogramming glucose metabolism.Communications biology · 2025Article
- Untargeted Metabolomic Identifies Potential Seasonal Biomarkers of Semen Quality in Duroc Boars.Biology · 2025Article
- Liquid chromatograph-mass spectrometry metabolomics uncovers potential biomarkers of semen cryo-injury in goats.Animal bioscience · 2025Article
- Blood plasma trimethylamine N-oxide and related metabolites and asthenozoospermia odds: a hospital-based matched case-control study in China.Human reproduction open · 2025Article
- Effects of Varying Light Durations on Sperm Quality in Rams.Animals : an open access journal from MDPI · 2024Article
- Seminal plasma metabolomics analysis of differences in liquid preservation ability of boar sperm.Journal of animal science · 2023Article
- Review
- Reducing the Glucose Level in Pre-treatment Solution Improves Post-thaw Boar Sperm Quality.Frontiers in veterinary science · 2022Article
- Metabolomic analysis reveals spermatozoa and seminal plasma differences between Duroc and Liang guang Small-spotted pig.Frontiers in veterinary science · 2022Article
- Metabolomics analysis of buck semen cryopreserved with trehalose.Frontiers in genetics · 2022Article
- Metabolomic fingerprinting of pig seminal plasma identifies in vivo fertility biomarkers.Journal of animal science and biotechnology · 2021Article
- Measurement of Oxidative Stress Index in Seminal Plasma Can Predict In Vivo Fertility of Liquid-Stored Porcine Artificial Insemination Semen Doses.Antioxidants (Basel, Switzerland) · 2021Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolomic approaches allow the study of downstream gene expression events since metabolites are considered as the products of cell signaling pathways. For this reason, many studies in humans have already been conducted to determine the influence of the metabolites present in seminal plasma (SP) on sperm physiology, and to identify putative biomarkers. However, in livestock species, these relationships are yet to be uncovered. Thus, the present study aimed to explore: (i) if concentrations of metabolites in pig SP are related to sperm quality and functionality, and (ii) if they could predict the sperm resilience to liquid storage at 17°C. To this end, 28 ejaculates were individually collected and split into three aliquots: one was used for SP analysis through nuclear magnetic resonance (NMR) spectroscopy; another served for the evaluation of sperm concentration and morphology; and the last one was utilized to determine sperm functionality parameters using computer-assisted sperm analysis (CASA) and flow cytometry after 0 h and 72 h of liquid-storage at 17°C. NMR analysis allowed the identification and quantification of 23 metabolites present in pig SP which, except for fumarate, were not observed to follow a breed-dependent behavior. Moreover, specific relationships between metabolites and sperm variables were identified: (i) glutamate, methanol, trimethylamine N-oxide, carnitine, and isoleucine were seen to be related to some sperm quality and functionality parameters evaluated immediately after semen collection; (ii) leucine, hypotaurine, carnitine and isoleucine were found to be associated to the sperm ability to withstand liquid storage; and (iii) Bayesian multiple regression models allowed the identification of metabolite patterns for specific sperm parameters at both 0 h and 72 h. The identification of these relationships opens up the possibility of further investigating these metabolites as potential sperm functional biomarkers.
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