ArticleFrontiers in cellular and infection microbiology2021
A Ligation/Recombinase Polymerase Amplification Assay for Rapid Detection of SARS-CoV-2.
Article in Frontiers in cellular and infection microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Recombinase Polymerase Amplification (RPA) as an Isothermal Molecular-Point of Care Test (IM-POCT) for Rapid Identification of P. aeruginosa Among Patients with Respiratory Infection.Molecular biotechnology · 2026Article
- Multiplexed Salivary miRNA Quantification for Predicting Severe COVID-19 Symptoms in Children Using Ligation-RPA Amplification Assay.ACS sensors · 2025Article
- Rolosense: Mechanical Detection of SARS-CoV-2 Using a DNA-Based Motor.ACS central science · 2024Article
- Advances and Challenges in SARS-CoV-2 Detection: A Review of Molecular and Serological Technologies.Diagnostics (Basel, Switzerland) · 2024Review
- Multiplex Detection of RNA Viruses Based on Ligation Reaction and Universal PCR Amplification.Current microbiology · 2024Article
- Rapid detection of EGFR mutation in CTCs based on a double spiral microfluidic chip and the real-time RPA method.Analytical and bioanalytical chemistry · 2023Article
- RT-nestRPA is a new technology for the rapid and sensitive detection of nucleic acid detection of pathogens used for a variety of medical application scenarios.Analytica chimica acta · 2023Article
- Rolosense: Mechanical detection of SARS-CoV-2 using a DNA-based motor.bioRxiv : the preprint server for biology · 2023Article
- COVID-19 diagnostics: Molecular biology to nanomaterials.Clinica chimica acta; international journal of clinical chemistry · 2023Review
- Current trends in COVID-19 diagnosis and its new variants in physiological fluids: Surface antigens, antibodies, nucleic acids, and RNA sequencing.Trends in analytical chemistry : TRAC · 2022Review
- Review
- A precise review on NAATs-based diagnostic assays for COVID-19: A motion in fast POC molecular tests.European journal of clinical investigation · 2022Review
- A homogeneous nucleic acid assay for simultaneous detection of SARS-CoV-2 and influenza A (H3N2) by single-particle inductively coupled plasma mass spectrometry.Analytica chimica acta · 2021Article
- Sample-to-answer salivary miRNA testing: New frontiers in point-of-care diagnostic technologies.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pandemic of COVID-19 caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has led to more than 117 million reported cases and 2.6 million deaths. Accurate diagnosis technologies are vital for controlling this pandemic. Reverse transcription (RT)-based nucleic acid detection assays have been developed, but the strict sample processing requirement of RT has posed obstacles on wider applications. This study established a ligation and recombinase polymerase amplification (L/RPA) combined assay for rapid detection of SARS-CoV-2 on genes N and ORF1ab targeting the specific biomarkers recommended by the China CDC. Ligase-based strategies usually have a low-efficiency problem on RNA templates. This study has addressed this problem by using a high concentration of the T4 DNA ligase and exploiting the high sensitivity of RPA. Through selection of the ligation probes and optimization of the RPA primers, the assay achieved a satisfactory sensitivity of 10
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