Evidence map›Paper›PMID 34123149›Full record

ArticleChemical science2020

Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad.

Andrew T Frawley, Virginia Wycisk, Yaoyao Xiong, Silvia Galiani, Erdinc Sezgin, Iztok Urbančič, Andreas Vargas Jentzsch, Kathryn G Leslie, Christian Eggeling, Harry L Anderson

Abstract read
In one paragraph

Article in Chemical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Subphthalocyanine-flipper dyads for selective membrane staining.Physical chemistry chemical physics : PCCP · 2024
    Article
  2. Review
  3. Article
  4. Molecular Machines For The Control Of Transmembrane Transport.Journal of the American Chemical Society · 2023
    Review
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Andrew T FrawleyDepartment of Chemistry, University of Oxford, Chemistry Research Laboratory Oxford OX1 3TA UK harry.anderson@chem.ox.ac.uk.ORCID https://orcid.org/0000-0002-4890-6683
Virginia WyciskDepartment of Chemistry, University of Oxford, Chemistry Research Laboratory Oxford OX1 3TA UK harry.anderson@chem.ox.ac.uk.
Yaoyao XiongDepartment of Chemistry, University of Oxford, Chemistry Research Laboratory Oxford OX1 3TA UK harry.anderson@chem.ox.ac.uk.
Silvia GalianiMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford Oxford OX3 9DS UK christian.eggeling@rdm.ox.ac.uk.
Erdinc SezginMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford Oxford OX3 9DS UK christian.eggeling@rdm.ox.ac.uk.ORCID https://orcid.org/0000-0002-4915-388X
Iztok UrbančičMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford Oxford OX3 9DS UK christian.eggeling@rdm.ox.ac.uk.ORCID https://orcid.org/0000-0003-3603-6585
Andreas Vargas JentzschDepartment of Chemistry, University of Oxford, Chemistry Research Laboratory Oxford OX1 3TA UK harry.anderson@chem.ox.ac.uk.ORCID https://orcid.org/0000-0003-1386-3174
Kathryn G LeslieDepartment of Chemistry, University of Oxford, Chemistry Research Laboratory Oxford OX1 3TA UK harry.anderson@chem.ox.ac.uk.ORCID https://orcid.org/0000-0002-6816-2845
Christian EggelingMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford Oxford OX3 9DS UK christian.eggeling@rdm.ox.ac.uk.ORCID https://orcid.org/0000-0002-3698-5599
Harry L AndersonDepartment of Chemistry, University of Oxford, Chemistry Research Laboratory Oxford OX1 3TA UK harry.anderson@chem.ox.ac.uk.ORCID https://orcid.org/0000-0002-1801-8132

Funding

Medical Research Council G0902418Medical Research Council MC_UU_00008/9Medical Research Council MC_UU_12010/9Medical Research Council MR/K01577X/1Wellcome Trust
6 · The paper itself

Abstract

Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm).

Identifiers

PMID34123149
PMCPMC8163400

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.