Evidence map›Paper›PMID 34122014›Full record

ArticleFrontiers in cellular neuroscience2021

All-Optical Electrophysiology in hiPSC-Derived Neurons With Synthetic Voltage Sensors.

Francesca Puppo, Sanaz Sadegh, Cleber A Trujillo, Martin Thunemann, Evan P Campbell, Matthieu Vandenberghe, Xiwei Shan, Ibrahim A Akkouh, Evan W Miller, Brenda L Bloodgood and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
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  5. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Francesca PuppoDepartment of Pediatrics/Rady Children's Hospital San Diego, School of Medicine, University of California, San Diego, La Jolla, CA, United States.
Sanaz SadeghDepartment of Neurosciences, University of California, San Diego, La Jolla, CA, United States.
Cleber A TrujilloDepartment of Pediatrics/Rady Children's Hospital San Diego, School of Medicine, University of California, San Diego, La Jolla, CA, United States.
Martin ThunemannDepartment of Biomedical Engineering, Boston University, Boston, MA, United States.
Evan P CampbellDivision of Biological Sciences, Section of Neurobiology, University of California, San Diego, La Jolla, CA, United States.
Matthieu VandenbergheDivision of Mental Health and Addiction, Oslo University Hospital, NORMENT, KG Jebsen Centre for Neurodevelopmental Disorders, Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
Xiwei ShanDepartment of Pediatrics/Rady Children's Hospital San Diego, School of Medicine, University of California, San Diego, La Jolla, CA, United States.
Ibrahim A AkkouhDivision of Mental Health and Addiction, Oslo University Hospital, NORMENT, KG Jebsen Centre for Neurodevelopmental Disorders, Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
Evan W MillerDepartment of Chemistry and Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, United States.
Brenda L BloodgoodDivision of Biological Sciences, Section of Neurobiology, University of California, San Diego, La Jolla, CA, United States.
Gabriel A SilvaDepartment of Bioengineering, University of California, San Diego, La Jolla, CA, United States.
Anders M DaleDepartment of Radiology, University of California, San Diego, La Jolla, CA, United States.
Gaute T EinevollFaculty of Science and Technology, Norwegian University of Life Sciences, Ås, Norway.
Srdjan DjurovicDepartment of Medical Genetics, Oslo University Hospital, Oslo, Norway.
Ole A AndreassenDivision of Mental Health and Addiction, Oslo University Hospital, NORMENT, KG Jebsen Centre for Neurodevelopmental Disorders, Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
Alysson R MuotriDepartment of Pediatrics/Rady Children's Hospital San Diego, School of Medicine, University of California, San Diego, La Jolla, CA, United States.
Anna DevorDepartment of Biomedical Engineering, Boston University, Boston, MA, United States.

Funding

MICROSCOPIC FOUNDATION OF MULTIMODAL HUMAN IMAGINGR01MH111359 · NIMH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DALE, ANDERS M, DEVOR, ANNA · 2016 to 2020
$5.6M
Interrogating Neuronal Membrane Potential Dynamics with Optical Voltage SensorsR01NS098088 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Evan Walker Miller · 2017 to 2026
$4.4M
Transparent neural interface for in vivo interrogation of human organoidsR21EY030727 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DEVOR, ANNA · 2019 to 2019
$456k
NEI NIH HHS R21 EY030727NIMH NIH HHS R01 MH111359NINDS NIH HHS R01 NS098088
6 · The paper itself

Abstract

Voltage imaging and "all-optical electrophysiology" in human induced pluripotent stem cell (hiPSC)-derived neurons have opened unprecedented opportunities for high-throughput phenotyping of activity in neurons possessing unique genetic backgrounds of individual patients. While prior all-optical electrophysiology studies relied on genetically encoded voltage indicators, here, we demonstrate an alternative protocol using a synthetic voltage sensor and genetically encoded optogenetic actuator that generate robust and reproducible results. We demonstrate the functionality of this method by measuring spontaneous and evoked activity in three independent hiPSC-derived neuronal cell lines with distinct genetic backgrounds.

Indexed as

BeRST-1optogeneticsphenotypingstem cellsvoltage imaging

Identifiers

PMID34122014
PMCPMC8193062

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.