Evidence map›Paper›PMID 34121129›Full record

ArticleJournal of rehabilitation medicine2021

Reliability and validity of a standardised ultrasound examination protocol to quantify vastus lateralis muscle.

Theresa Maria Betz, Michaela Wehrstein, Fabian Preisner, Martin Bendszus, Birgit Friedmann-Bette

Abstract read
In one paragraph

Article in Journal of rehabilitation medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. The role of ultrasound of the quadriceps femoris in people with cystic fibrosis: a systematic review and meta-analysis.Jornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e Tisilogia · 2026
    Pooled it
  2. Review
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  4. A single day of reduced activity alters the next day's transcriptomic and metabolic exercise response.American journal of physiology. Endocrinology and metabolism · 2026
    Article
  5. [Emergency reconstruction of traumatic dynamic muscle defects using functional superficial vastus lateralis chimeric perforator flap based on descending branch of lateral femoral circumflex artery].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2025
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  18. Human skeletal muscle size with ultrasound imaging: a comprehensive review.Journal of applied physiology (Bethesda, Md. : 1985) · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Theresa Maria BetzCardiology, Angiology, Pneumonology, Medical Clinic, University Hospital of Heidelberg, Heidelberg, Germany. E-mail: theresamaria.betz@med.uni-heidelberg.de.
Michaela Wehrstein
Fabian Preisner
Martin Bendszus
Birgit Friedmann-Bette

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo evaluate the reliability and validity of a standardized ultrasound examination protocol for measuring vastus lateralis muscle size.

designProspective cohort study. SUBJECTS: Sixteen staff members of the university hospital of Heidelberg.

methodsMuscle thickness, cross-sectional area and subcutaneous adipose tissue thickness were measured at 3 standardized sites on the right and left vastus lateralis muscle. Ultrasound measurements were collected by 2 independent investigators on 2 different days and compared with magnetic resonance imaging measurements.

resultsIntraclass correlation coefficients (ICC) for intra- and inter-rater reliability showed very good closeness of agreement for all parameters (ICC = 0.929-0.994, p < 0.001). Muscle thickness and subcutaneous adipose tissue thickness ultrasound and magnetic resonance imaging measurements revealed good to very good closeness of agreement (ICC = 0.835-0.969, p < 0.001), whereas cross-sectional area showed only average closeness of agreement (ICC = 0.727, p < 0.001). A strong predictive positive correlation for ultrasound and magnetic resonance imaging-based measurements of cross-sectional area was found (R² = 0.793, p < 0.001).

conclusionBy standardization of an examination protocol, quantitative vastus lateralis muscle ultrasound proved to be a reliable method for assessing vastus lateralis muscle size. Furthermore, this protocol is valid for measuring muscle thickness and subcutaneous adipose tissue thickness, although there seems to be a systematic underestimation of cross-sectional area depending on subcutaneous adipose tissue thickness.

Indexed as

Clinical ProtocolsHumansMagnetic Resonance ImagingMaleProspective StudiesQuadriceps MuscleReproducibility of ResultsSubcutaneous FatUltrasonographydiagnostic imaginghypertrophymagnetic resonance imagingquadriceps musclesarcopaeniaskeletal musclesubcutaneous fatultrasound

Identifiers

PMID34121129
PMCPMC8638746

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.