ArticleBiosensors & bioelectronics2021
A CRISPR-based and post-amplification coupled SARS-CoV-2 detection with a portable evanescent wave biosensor.
Article in Biosensors & bioelectronics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Biosensors as transformative tools for multiplex detection of respiratory viral pathogens.Archives of microbiology · 2026Review
- Magnesium Oxide Nanoparticles Loaded with 6-Gingerol: A Bioinspired Approach to Anticancer, Anti-Inflammatory, and Antioxidant Therapy.International journal of nanomedicine · 2026Article
- CRISPR/Cas12a2 enables ultra-sensitive amplification-free RNA detection.Nucleic acids research · 2025Article
- MoSBiosensors · 2025Article
- Dual Mode Analysis of Methicillin-ResistantACS omega · 2025Article
- One-Step RAA and CRISPR-Cas13a Method for Detecting Influenza B Virus.Microbial biotechnology · 2025Article
- Recent Advances in the CRISPR/Cas-Based Nucleic Acid Biosensor for Food Analysis: A Review.Foods (Basel, Switzerland) · 2024Review
- Detection and isolation of brain tumors in cancer patients using neural network techniques in MRI images.Scientific reports · 2024Article
- Harnessing the power of clustered regularly interspaced short palindromic repeats (CRISPR) based microfluidics for next-generation molecular diagnostics.Molecular biology reports · 2024Review
- RNA-Based Sensor Systems for Affordable Diagnostics in the Age of Pandemics.ACS synthetic biology · 2024Review
- Combination of nucleic acid amplification and CRISPR/Cas technology in pathogen detection.Frontiers in microbiology · 2024Review
- Fabrication of MIL-101(Cr)/silver nanocomposites as SERS substrate for sensitive determination of malachite green and crystal violet in tilapia.Mikrochimica acta · 2023Article
- Biomedical equipments, vaccine and drug in the prevention, diagnosis and treatment of COVID-19.Heliyon · 2023Review
- A SERS-signalled, CRISPR/Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a single tube-in-tube vessel.Journal of hazardous materials · 2023Article
- An ultra-sensitive one-pot RNA-templated DNA ligation rolling circle amplification-assisted CRISPR/Cas12a detector assay for rapid detection of SARS-CoV-2.Biosensors & bioelectronics · 2023Article
- Application of Hybridization Chain Reaction/CRISPR-Cas12a for the Detection of SARS-CoV-2 Infection.Diagnostics (Basel, Switzerland) · 2023Article
- Conductive Ink-Coated Paper-Based Supersandwich DNA Biosensor for Ultrasensitive Detection ofBiosensors · 2023Article
- Recent Progress in Functional-Nucleic-Acid-Based Fluorescent Fiber-Optic Evanescent Wave Biosensors.Biosensors · 2023Review
- MXene-based composites against antibiotic-resistant bacteria: current trends and future perspectives.RSC advances · 2023Review
- Advances in the application of CRISPR-Cas technology in rapid detection of pathogen nucleic acid.Frontiers in molecular biosciences · 2023Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The continuing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, which causes coronavirus disease 2019 (COVID-19), has spread globally and its reliable diagnosis is one of the foremost priorities for protecting public health. Herein a rapid (<1 h), easy-to-implement, and accurate CRISPR-based evanescent wave fluorescence biosensing platform for detection of SARS-CoV-2 is reported. The collateral effect of Cas13a is combined with a universal autonomous enzyme-free hybridization chain reaction (HCR) by designing a cleavage hairpin reporter, which is cleaved upon target recognition, and hence releasing the initiator sequence to trigger the downstream HCR circuits. Detection of HCR assemblies is accomplished by first adsorbing to the desthiobiotin-modified optical fiber, followed by fluorescence emission induced by an evanescent field. Three Cas13a crRNAs targeting the genes of S, N and Orf1ab of SARS-CoV-2 are programmed to specifically target SARS-CoV-2 or broadly detect related coronavirus strains, such as MERS-CoV and SARS-CoV. The HCR amplification coupled Cas13a-based biosensing platform is capable of rapid detection of SARS-CoV-2 with attomolar sensitivity. This method is further validated by adding target RNA of SARS-CoV-2 in negative oropharyngeal swabs. The good discrimination capability of this technique demonstrates its promising potential for point-of-care diagnosis of COVID-19.
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