Evidence map›Paper›PMID 34119483›Full record

ArticleExperimental eye research2021

The aging mouse lens transcriptome.

Adam P Faranda, Mahbubul H Shihan, Yan Wang, Melinda K Duncan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 1 pooled it
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.

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  19. Modeling Cataract Surgery in Mice.Journal of visualized experiments : JoVE · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Adam P FarandaDepartment of Biological Sciences University of Delaware Newark, DE, 19716, USA.
Mahbubul H ShihanDepartment of Biological Sciences University of Delaware Newark, DE, 19716, USA.
Yan WangDepartment of Biological Sciences University of Delaware Newark, DE, 19716, USA.
Melinda K DuncanDepartment of Biological Sciences University of Delaware Newark, DE, 19716, USA. Electronic address: duncanm@udel.edu.
University of Delaware · US

Funding

Predictive Modeling & Optimal Control Framework for Model-Based Epidemic Response in DelawareP20GM103446 · NIGMS · UNIVERSITY OF DELAWARE · PI MELINDA K DUNCAN · 2012 to 2026
$67.2M
The mechanisms underlying posterior capsular opacificationR01EY028597 · NEI · UNIVERSITY OF DELAWARE · PI DUNCAN, MELINDA K · 2018 to 2021
$1.5M
Acquisition of a Zeiss LSM710 Confocal MicroscopeS10RR027273 · NCRR · UNIVERSITY OF DELAWARE · PI DUNCAN, MELINDA K · 2010 to 2010
$494k
NCRR NIH HHS S10 RR027273NEI NIH HHS R01 EY028597NIGMS NIH HHS P20 GM103446
6 · The paper itself

Abstract

Age is a major risk factor for cataract (ARC). However, the influence of aging on the lens transcriptome is under studied. Lens epithelial (LEC) and fiber cells (LFC) were isolated from young (3 month old) and aged (24 month old) C57BL/6J mice, and the transcriptome elucidated via RNAseq. EdgeR estimated differential gene expression in pairwise contrasts, and Advaita's Ipathway guide and custom R scripts were used to evaluate the potential biological significance of differentially expressed genes (DEGs). This analysis revealed age-dependent decreases in lens differentiation marker expression in both LECs and LFCs, with gamma crystallin transcripts downregulating nearly 50 fold in aged LFCs. The expression of the transcription factors Hsf4 and Maf, which are known to activate lens fiber cell preferred genes, are downregulated, while FoxE3, which represses gamma crystallin expression, is upregulated in aged fibers. Aged LECs upregulate genes controlling the immune response, complement pathways, and cellular stress responses, including glutathione peroxidase 3 (Gpx3). Aged LFCs exhibit broad changes in the expression of genes regulating cell communication, and upregulate genes involved in antigen processing/presentation and cholesterol metabolism, while changes in the expression of mitochondrial respiratory chain genes are consistent with mitochondrial stress, including upregulation of NDufa4l2, which encodes an alternate electron transport chain protein. However, age did not profoundly affect the response of LECs to injury as both young and aged LECs upregulate inflammatory gene signatures at 24 h post injury to similar extents. These RNAseq profiles provide a rich data set that can be mined to understand the genetic regulation of lens aging and how this impinges on the pathophysiology of age related cataract.

Indexed as

Gene Expression RegulationAgingAnimalsCataractDisease Models, AnimalFemaleForkhead Transcription Factorsgamma-CrystallinsHeat-Shock ProteinsHeat Shock Transcription FactorsLens, CrystallineMaleMiceMice, Inbred C57BLProto-Oncogene Proteins c-mafRNAForkhead Transcription FactorsFoxe3 protein, mousegamma-CrystallinsHeat-Shock ProteinsHeat Shock Transcription FactorsHsf4 protein, mouseMaf protein, mouseProto-Oncogene Proteins c-mafRNAAgingCholesteroComplement pathwaysLensMetabolismPosterior capsular opacificationSenescenceTranscriptome

Identifiers

PMID34119483
PMCPMC8720273
OpenAlexW3167746770

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.