ArticleAnalytica chimica acta2021
One-step enzyme kinetics measurement in 3D printed microfluidics devices based on a high-performance single vibrating sharp-tip mixer.
Article in Analytica chimica acta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Recent advances in 3D printing for biochemical and environmental analysis.Mikrochimica acta · 2026Review
- Advances in Machine Learning Models for Predicting Enzyme Kinetic Parameters.Journal of chemical information and modeling · 2026Review
- Recent Advances in Microfluidic Biofuel Cells.Biosensors · 2025Review
- Acoustofluidics: Technology Advances and Applications from 2022 to 2024.Analytical chemistry · 2025Review
- Microfluidic systems and ultrasonics for emulsion-based biopolymers: A comprehensive review of techniques, challenges, and future directions.Ultrasonics sonochemistry · 2025Review
- 3D Printing in Biocatalysis and Biosensing: From General Concepts to Practical Applications.Chemistry, an Asian journal · 2024Review
- Enzymatic Electrochemical/Fluorescent Nanobiosensor for Detection of Small Chemicals.Biosensors · 2023Review
- Microfluidic Continuous Flow DNA Fragmentation based on a Vibrating Sharp-tip.Microfluidics and nanofluidics · 2022Article
- A Lotus shaped acoustofluidic mixer: High throughput homogenisation of liquids in 2 ms using hydrodynamically coupled resonators.Ultrasonics sonochemistry · 2022Article
- PDMS Microfabrication and Design for Microfluidics and Sustainable Energy Application: Review.Micromachines · 2021Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Measuring enzyme kinetics is of great importance to understand many biological processes and improve biosensing and industrial applications. Conventional methods of measuring enzyme kinetics require to prepare a series of solutions with different substrate concentrations and measure the signal response over time with these solutions, leading to tedious sample preparation steps, high reagents/sample consumption, and difficulties in studying fast enzyme kinetics. Here we report a one-step assay to measure enzyme kinetics using a 3D-printed microfluidic device, which eliminates the steps of preparing and handling multiple solutions thereby simplifying the whole workflow significantly. The assay is enabled by a highly efficient vibrating sharp-tip mixing method that can mix multiple streams of fluids with minimal mixing length (∼300 μm) and time (as low as 3 ms), and a wide range of working flow rates from 1.5 μL/min to 750 μL/min. Owing to the high performance of the mixer, a series of experiments with different substrate concentrations are performed by simply adjusting the flow rates of reagents loaded from three inlets in one experiment run. The Michaelis-Menten kinetics of the horseradish peroxidase (HRP)-catalyzed reaction between H
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Registered trials
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