ArticlePlant molecular biology2021
The interaction of MYB, bHLH and WD40 transcription factors in red pear (Pyrus pyrifolia) peel.
Article in Plant molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 66 citations in OpenAlex.
- Evolutionary and functional divergence of MYB regulatory networks in flavonoid biosynthesis across Poaceae, non-Poaceae monocots, and eudicot horticultural plants.Horticulture research · 2026Review
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- Anthocyanin biosynthesis in blueberry is regulated by light and abscisic acid signaling via a VcABF2-miR156-VcSPL9 feedback loop.The New phytologist · 2026Article
- Genome-wide identification of the MYB gene family and identification of candidate genes that involved in anthocyanin synthesis in cowpea (Vigna unguiculata [L.] Walp).BMC plant biology · 2026Article
- Molecular insights into the regulation of flavonoid biosynthesis in fruits.Horticulture research · 2026Article
- Improving drought stress resilience in grapevine using Aspergillus niger strain ZRS14 under in vitro conditions.Plant cell reports · 2025Article
- T and Z, partial seed coat patterning genes in common bean, provide insight into the structure and protein interactions of a plant MBW complex.G3 (Bethesda, Md.) · 2024Article
- A Functional InDel in the WRKY10 Promoter Controls the Degree of Flesh Red Pigmentation in Apple.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The R2R3-MYB transcription factor ZeMYB32 negatively regulates anthocyanin biosynthesis in Zinnia elegans.Plant molecular biology · 2024Article
- Multi-Omics Research Accelerates the Clarification of the Formation Mechanism and the Influence of Leaf Color Variation in Tea (Plants (Basel, Switzerland) · 2024Review
- Integrative metabolome and transcriptome analyses reveal the coloration mechanism in Camellia oleifera petals with different color.BMC plant biology · 2024Article
- Article
- Unveiling the protective role of anthocyanin in rice: insights into drought-induced oxidative stress and metabolic regulation.Frontiers in plant science · 2024Article
- Cloned genes and genetic regulation of anthocyanin biosynthesis in maize, a comparative review.Frontiers in plant science · 2024Review
- Genome-Wide Identification, Evolution, and Expression Analysis of the WD40 Subfamily inInternational journal of molecular sciences · 2023Article
- FaMYB5 Interacts with FaBBX24 to Regulate Anthocyanin and Proanthocyanidin Biosynthesis in Strawberry (International journal of molecular sciences · 2023Article
- The Role of Anthocyanins in Plant Tolerance to Drought and Salt Stresses.Plants (Basel, Switzerland) · 2023Review
- ETHYLENE RESPONSE FACTORS 4.1/4.2 with an EAR motif repress anthocyanin biosynthesis in red-skinned pears.Plant physiology · 2023Article
- Involvement of a MYB Transcription Factor in Anthocyanin Biosynthesis during Chinese Bayberry (Biology · 2023Article
- The Splicing Factor SR45 Negatively Regulates Anthocyanin Accumulation under High-Light Stress inLife (Basel, Switzerland) · 2023Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
key messageSunlight enhanced peel color and significantly up-regulated the expression of PyMYB10 and PybHLH genes. MYB-bHLH-WD40 transcriptional complex forms in the light and is involved in regulating anthocyanin accumulation in the peel. Anthocyanin is the major pigment in the peel of Yunnan red pear (Pyrus pyrifolia (Burm.) Nak.). A transcriptional activation protein complex, involving members of the transcription factor classes of MYB, bHLH and WD40, regulates anthocyanin biosynthesis. This complex was examined in the peel of red pear. In order to clarify the interaction of PyMYB10, PybHLH and PyWD40, fruit were bagged then peel samples collected 0, 3, 5, and 7 days after bag removal. Samples were used for Western blotting and protein interaction analysis. The results showed that sunlight enhanced peel color and significantly up-regulated the expression of both PyMYB10 and PybHLH genes. Co-immunoprecipitation (Co-IP) analysis showed that PybHLH interacted with PyMYB10 or PyWD40, and PyMYB10 interacted with PyWD40. Using onion cells as a model system, bimolecular fluorescence complementation (BiFC) confirmed these interactions and showed that the interaction localized to the nuclei. GST Pull down and Far-Western blotting assays demonstrated that PybHLH interacted with PyMYB10 or PyWD40, respectively, and PyMYB10 interacted with PyWD40 in vitro. In addition, EMSA assay showed that PyMYB10 can directly bind to the promoter of the gene encoding the anthocyanin biosynthesis enzyme anthocyanidin synthase (PyANS). Taken together, these results showed that the ternary complex of PyMYB10, PybHLH and PyWD40 transcription factors forms to regulate anthocyanin biosynthesis and accumulation in Yunnan red pear.
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