ArticleCommunications biology2021
Phasor-based hyperspectral snapshot microscopy allows fast imaging of live, three-dimensional tissues for biomedical applications.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Medical hyperspectral imaging: an updated review of technology advancements and biomedical applications.Journal of biomedical optics · 2026Pooled it
- Python-controlled multimodal UV-VIS hyperspectral imaging system.Journal of biomedical optics · 2026Article
- Phasor-Based Spatio-Spectral Segmentation for Hyperspectral Fluorescence Microscopy.Journal of biophotonics · 2026Article
- Deep unfolding blind source unmixing for multicolor fluorescence imaging.Biomedical optics express · 2026Article
- Multimodal Phasor Analysis for Digital Pathology: Quantitative Characterization of Liver Iron Overload.Chemical & biomedical imaging · 2026Article
- High-Throughput Hyperspectral and Multiplexed Super-Resolution Fluorescence Imaging by SP-STORM.JACS Au · 2026Article
- Evaluating Preimplantation Embryo Metabolism Through Imaging of Cellular Autofluorescence.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Redefining colocalization analysis with a novel phasor mixing coefficient.Journal of cell science · 2026Article
- Spectral phasor imaging on a commercial confocal microscope without a spectral detector.Scientific reports · 2025Article
- Unlocking the potential of large-scale 3D imaging with tissue clearing techniques.Microscopy (Oxford, England) · 2025Review
- Imaging 3D cell cultures with optical microscopy.Nature methods · 2025Review
- High-Speed Hyperspectral Imaging for Near Infrared Fluorescence and Environmental Monitoring.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Multiphoton microscopy at a microwatt level via gain-managed nonlinear amplification and pulse-picking.Biomedical optics express · 2025Article
- Multimodal esophageal cancer imaging: establishing data processing techniques and assessing diagnostic sensitivity.Biophotonics discovery · 2025Article
- Advancing hyperspectral imaging and machine learning tools toward clinical adoption in tissue diagnostics: A comprehensive review.APL bioengineering · 2024Review
- Advancements in Hyperspectral Imaging and Computer-Aided Diagnostic Methods for the Enhanced Detection and Diagnosis of Head and Neck Cancer.Biomedicines · 2024Review
- Snapshot spectral imaging: from spatial-spectral mapping to metasurface-based imaging.Nanophotonics (Berlin, Germany) · 2024Review
- More than double the fun with two-photon excitation microscopy.Communications biology · 2024Review
- Multiplexed imaging in live cells using pulsed interleaved excitation spectral FLIM.Optics express · 2024Article
- Imagining the future of optical microscopy: everything, everywhere, all at once.Communications biology · 2023Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Hyperspectral imaging is highly sought after in many fields including mineralogy and geology, environment and agriculture, astronomy and, importantly, biomedical imaging and biological fluorescence. We developed ultrafast phasor-based hyperspectral snapshot microscopy based on sine/cosine interference filters for biomedical imaging not feasible with conventional hyperspectral detection methods. Current approaches rely on slow spatial or spectral scanning limiting their application in living biological tissues, while faster snapshot methods such as image mapping spectrometry and multispectral interferometry are limited in spatial and/or spectral resolution, are computationally demanding, and imaging devices are very expensive to manufacture. Leveraging light sheet microscopy, phasor-based hyperspectral snapshot microscopy improved imaging speed 10-100 fold which, combined with minimal light exposure and high detection efficiency, enabled hyperspectral metabolic imaging of live, three-dimensional mouse tissues not feasible with other methods. As a fit-free method that does not require any a priori information often unavailable in complex and evolving biological systems, the rule of linear combinations of the phasor could spectrally resolve subtle differences between cell types in the developing zebrafish retina and spectrally separate and track multiple organelles in 3D cultured cells over time. The sine/cosine snapshot method is adaptable to any microscope or imaging device thus making hyperspectral imaging and fit-free analysis based on linear combinations broadly available to researchers and the public.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.