ArticleScientific reports2021
Development of a capture sequencing assay for enhanced detection and genotyping of tick-borne pathogens.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Xenodiagnosis to Search for Borrelia burgdorferi After Antibiotic Treatment of Lyme Disease: A Prospective Cohort Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2026Trial
- Two Coding-Complete Genomes of Tick-Borne Encephalitis Virus Sequenced fromPathogens (Basel, Switzerland) · 2026Article
- Adaptation of custom capture sequencing panels to the Oxford Nanopore MinION platform.Molecular biology reports · 2026Article
- Dermacentor occidentalis Ticks and Link to Rickettsia lanei Infections, California, USA.Emerging infectious diseases · 2026Article
- The multiplexed single-tier InBios Lyme Detect Multiplex ELISA is more sensitive than standard two-tier tests in the early stages of Lyme disease.Journal of clinical microbiology · 2025Article
- Evaluating methods for genome sequencing ofMicrobial genomics · 2025Article
- Tailored Functionalization of Plasmonic AgNPs/C:H:N:O Nanocomposite for Sensitive and Selective Detection.Journal of biophotonics · 2025Article
- Research progress on diagnostic techniques for differentFrontiers in cellular and infection microbiology · 2025Review
- Development of Multiplex Assays for the Identification of ZoonoticPathogens (Basel, Switzerland) · 2024Article
- Empirical assessment of the enrichment-based metagenomic methods in identifying diverse respiratory pathogens.Scientific reports · 2024Article
- Turning the needle into the haystack: Culture-independent amplification of complex microbial genomes directly from their native environment.PLoS pathogens · 2024Review
- Human Babesia odocoilei and Bartonella spp. co-infections in the Americas.Parasites & vectors · 2024Article
- Retrospective Analysis of Potential Lyme Disease Clinical Cases in Argentina.Microorganisms · 2024Article
- Hybrid-Capture Target Enrichment in Human Pathogens: Identification, Evolution, Biosurveillance, and Genomic Epidemiology.Pathogens (Basel, Switzerland) · 2024Review
- Update on tick-borne pathogens detection methods within ticks.Current research in parasitology & vector-borne diseases · 2024Article
- Tick-Borne Co-Infections: Challenges in Molecular and Serologic Diagnoses.Pathogens (Basel, Switzerland) · 2023Review
- Multiple piroplasm parasites (Apicomplexa: Piroplasmida) in northeastern populations of the invasive Asian longhorned tick,Parasitology · 2023Article
- BosR and PlzA reciprocally regulate RpoS function to sustain Borrelia burgdorferi in ticks and mammals.The Journal of clinical investigation · 2023Article
- Genomic characterization of Francisella tularensis and other diverse Francisella species from complex samples.PloS one · 2022Article
- Capture Sequencing Enables Sensitive Detection of Tick-Borne Agents in Human Blood.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
Inadequate sensitivity has been the primary limitation for implementing high-throughput sequencing for studies of tick-borne agents. Here we describe the development of TBDCapSeq, a sequencing assay that uses hybridization capture probes that cover the complete genomes of the eleven most common tick-borne agents found in the United States. The probes are used for solution-based capture and enrichment of pathogen nucleic acid followed by high-throughput sequencing. We evaluated the performance of TBDCapSeq to surveil samples that included human whole blood, mouse tissues, and field-collected ticks. For Borrelia burgdorferi and Babesia microti, the sensitivity of TBDCapSeq was comparable and occasionally exceeded the performance of agent-specific quantitative PCR and resulted in 25 to > 10,000-fold increase in pathogen reads when compared to standard unbiased sequencing. TBDCapSeq also enabled genome analyses directly within vertebrate and tick hosts. The implementation of TBDCapSeq could have major impact in studies of tick-borne pathogens by improving detection and facilitating genomic research that was previously unachievable with standard sequencing approaches.
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