Evidence map›Paper›PMID 34115787›Full record

ArticlePloS one2021

Genome skimming reveals novel plastid markers for the molecular identification of illegally logged African timber species.

Maurizio Mascarello, Mario Amalfi, Pieter Asselman, Erik Smets, Olivier J Hardy, Hans Beeckman, Steven B Janssens

Abstract read
In one paragraph

Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Characterization of the complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2026
    Article
  3. The complete chloroplast genome and phylogenetic analysis ofMitochondrial DNA. Part B, Resources · 2026
    Article
  4. The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2026
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2023
    Article
  10. Article
  11. Article
  12. Article
  13. The complete chloroplast genome sequence ofMitochondrial DNA. Part B, Resources · 2023
    Article
  14. New Insights Into The Evolution of Chloroplast Genomes inEvolutionary bioinformatics online · 2023
    Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Maurizio MascarelloMeise Botanic Garden, Meise, Belgium.ORCID 0000-0003-0325-2345
Mario AmalfiMeise Botanic Garden, Meise, Belgium.
Pieter AsselmanMycology & Systematic and Evolutionary Botany, Department of Biology, Ghent University, Ghent, Belgium.ORCID 0000-0003-3491-2174
Erik SmetsNaturalis Biodiversity Center, Leiden, The Netherlands.
Olivier J HardyEvolutionary Biology and Ecology, Université Libre de Bruxelles, Brussels, Belgium.
Hans BeeckmanWood Biology, Department of Biology, Royal Museum for Central Africa, Tervuren, Belgium.
Steven B JanssensMeise Botanic Garden, Meise, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tropical forests represent vast carbon stocks and continue to be key carbon sinks and buffer climate changes. The international policy constructed several mechanisms aiming at conservation and sustainable use of these forests. Illegal logging is an important threat of forests, especially in the tropics. Several laws and regulations have been set up to combat illegal timber trade. Despite significant enforcement efforts of these regulations, illegal logging continues to be a serious problem and impacts for the functioning of the forest ecosystem and global biodiversity in the tropics. Microscopic analysis of wood samples and the use of conventional plant DNA barcodes often do not allow to distinguish closely-related species. The use of novel molecular technologies could make an important contribution for the identification of tree species. In this study, we used high-throughput sequencing technologies and bioinformatics tools to obtain the complete de-novo chloroplast genome of 62 commercial African timber species using the genome skimming method. Then, we performed a comparative genomic analysis that revealed new candidate genetic regions for the discrimination of closely-related species. We concluded that genome skimming is a promising method for the development of plant genetic markers to combat illegal logging activities supporting CITES, FLEGT and the EU Timber Regulation.

Indexed as

PlastidsAfricaConservation of Natural ResourcesDNA Barcoding, TaxonomicForestsGenetic MarkersGenome, ChloroplastHigh-Throughput Nucleotide SequencingPhylogenyTreesGenetic Markers

Identifiers

PMID34115787
PMCPMC8195358

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.