ArticleJournal, genetic engineering & biotechnology2021
Multiplex CRISPR/Cas9-mediated genome editing of the FAD2 gene in rice: a model genome editing system for oil palm.
Article in Journal, genetic engineering & biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Prospects and challenges in using engineered lactic acid bacteria in aquaculture applications.Engineering microbiology · 2026Review
- Transcriptional and metabolic analysis of oleic acid synthesis in seedless and tenera oil palm species.Frontiers in plant science · 2025Article
- Review
- Transcriptional engineering for value enhancement of oilseed crops: a forward perspective.Frontiers in genome editing · 2024Review
- Multiplex CRISPR/Cas9 gene-editing platform in oil palm targeting mutations in EgFAD2 and EgPAT genes.Journal, genetic engineering & biotechnology · 2023Article
- CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops.Frontiers in plant science · 2023Review
- Oil Palm Breeding in the Modern Era: Challenges and Opportunities.Plants (Basel, Switzerland) · 2022Review
- High-throughput methods for genome editing: the more the better.Plant physiology · 2022Review
- Micro-RNA-RegulatedPlants (Basel, Switzerland) · 2022Article
- Is CRISPR/Cas9 a way forward to fast-track genetic improvement in commercial palms? Prospects and limits.Frontiers in plant science · 2022Review
- CRISPR/Cas9-Targeted Mutagenesis ofFrontiers in plant science · 2022Article
- Applications and prospects of genome editing in plant fatty acid and triacylglycerol biosynthesis.Frontiers in plant science · 2022Review
- CRISPR/Cas9-mediated editing of double loci ofFrontiers in plant science · 2022Article
- C-to-G Base Editing Enhances Oleic Acid Production by Generating Novel Alleles ofFrontiers in plant science · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGenome editing employing the CRISPR/Cas9 system has been widely used and has become a promising tool for plant gene functional studies and crop improvement. However, most of the applied CRISPR/Cas9 systems targeting one locus using a sgRNA resulted in low genome editing efficiency.
resultsHere, we demonstrate the modification of the FAD2 gene in rice using a multiplex sgRNA-CRISPR/Cas9 genome editing system. To test the system's efficiency for targeting multiple loci in rice, we designed two sgRNAs based on FAD2 gene sequence of the Oryza sativa Japonica rice. We then inserted the validated sgRNAs into a CRISPR/Cas9 basic vector to construct pYLCRISPRCas9PUbi-H:OsFAD2. The vector was then transformed into protoplast cells isolated from rice leaf tissue via PEG-mediated transfection, and rice calli using biolistic transformation. Direct DNA sequencing of PCR products revealed mutations consisting of deletions of the DNA region between the two target sgRNAs.
conclusionThe results suggested that the application of the multiplex sgRNA-CRISPR/Cas9 genome editing system may be useful for crop improvement in monocot species that are recalcitrant to genetic modification, such as oil palm.
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