Evidence map›Paper›PMID 34115123›Full record

ArticleGenome biology and evolution2021

Genome Assembly of the Cold-Tolerant Leaf Beetle Gonioctena quinquepunctata, an Important Resource for Studying Its Evolution and Reproductive Barriers between Species.

Svitlana Lukicheva, Jean-François Flot, Patrick Mardulyn

Abstract read
In one paragraph

Article in Genome biology and evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Svitlana LukichevaEvolutionary Biology and Ecology & Interuniversity Institute of Bioinformatics in Brussels - (IB), Université Libre de Bruxelles (ULB), Brussels, Belgium.ORCID 0000-0003-0170-6868
Jean-François FlotEvolutionary Biology and Ecology & Interuniversity Institute of Bioinformatics in Brussels - (IB), Université Libre de Bruxelles (ULB), Brussels, Belgium.
Patrick MardulynEvolutionary Biology and Ecology & Interuniversity Institute of Bioinformatics in Brussels - (IB), Université Libre de Bruxelles (ULB), Brussels, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Coleoptera is the most species-rich insect order, yet is currently underrepresented in genomic databases. An assembly was generated for ca. 1.7-Gb genome of the leaf beetle Gonioctena quinquepunctata by first assembling long-sequence reads (Oxford Nanopore; ± 27-fold coverage) and subsequently polishing the resulting assembly with short sequence reads (Illumina; ± 85-fold coverage). The unusually large size (most Coleoptera species are associated with a reported size below 1 Gb) was at least partially attributed to the presence of a large fraction of repeated elements (73.8%). The final assembly was characterized by an N50 length of 432 kb and a BUSCO score of 95.5%. The heterozygosity rate was ±0.6%. Automated genome annotation informed by RNA-Seq resulted in 40,568 predicted proteins, which is much larger than the typical range 17,000-23,000 predicted for other Coleoptera. However, no evidence of a genome duplication was detected. This new reference genome will contribute to our understanding of genetic variation in the Coleoptera. Among others, it will also allow exploring reproductive barriers between species, investigating introgression in the nuclear genome, and identifying genes involved in resistance to extreme climate conditions.

Indexed as

ColeopteraNanoporesAnimalsGenomeHigh-Throughput Nucleotide SequencingMolecular Sequence AnnotationChrysomelidaede novo assemblygenome annotationwhole-genome sequence

Identifiers

PMID34115123
PMCPMC8290105

What OpenQuestion holds

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