ArticleInternational journal of legal medicine2021
GFAP positivity in neurons following traumatic brain injuries.
Article in International journal of legal medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 3 of them syntheses that pooled it.
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Who cites it
31 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- The Role of GFAP in Post-Mortem Analysis of Traumatic Brain Injury: A Systematic Review.International journal of molecular sciences · 2024Pooled it
- Pooled it
- GFAP and UCHL1 in Non-traumatic SAH: The Story thus Far. A Systematic Review of the Literature.CNS & neurological disorders drug targets · 2024Pooled it
- Aspiration Patch Proteomics Enables CE-ESI-MS Proteotyping of Identified Single Neurons in Intact Brain Tissue.Analytical chemistry · 2026Article
- Association of Preinjury Beta-Blocker Exposure With Brain Injury Biomarkers Following Traumatic Brain Injury.Journal of neurosurgical anesthesiology · 2026Article
- Reversibly unlocking the blood-brain barrier: A study on microtubule dynamics modulation using gold nanoparticle-modified reduced graphene oxide.Acta pharmaceutica Sinica. B · 2026Article
- Hyperbaric oxygen therapy alleviates intestinal and brain damage in experimental necrotizing enterocolitis.Medical gas research · 2025Article
- Histological and Immunohistochemical Analysis of the Mouse Brain Following Traumatic Brain Injury: Identification of Therapeutic Targets and Establishment of Treatment Windows.Bulletin of experimental biology and medicine · 2025Article
- Postmortem biochemistry of GFAP, NSE and S100B in cerebrospinal fluid and in vitreous humor for estimation of postmortem interval: a pilot study.Forensic science, medicine, and pathology · 2025Article
- Potential Correlation Between Molecular Biomarkers and Oxidative Stress in Traumatic Brain Injury.International journal of molecular sciences · 2025Review
- SGK1 upregulation in GFAPCell death & disease · 2025Article
- Article
- Disentangling relationships between Alzheimer's disease plasma biomarkers and established biomarkers in patients of tertiary memory clinics.EBioMedicine · 2025Article
- Plasma glial fibrillary acidic protein as a biomarker of blood-brain barrier integrity in patients with occult cerebral small vessel disease.American journal of translational research · 2025Article
- Posthospitalization COVID-19 cognitive deficits at 1 year are global and associated with elevated brain injury markers and gray matter volume reduction.Nature medicine · 2025Article
- Subregional differences in the hippocampal transcriptomic response after penetrating traumatic brain injury in rats.Frontiers in neurology · 2025Article
- Traumatic Brain Injury as a Public Health Issue: Epidemiology, Prognostic Factors and Useful Data from Forensic Practice.Healthcare (Basel, Switzerland) · 2024Review
- Development of Tissue-Engineered Model of Fibrotic Scarring after Spinal Cord Injury to Study Astrocyte Activation and Neurite Outgrowth In Vitro.ACS biomaterials science & engineering · 2024Article
- Confounding factors of the expression of mTBI biomarkers, S100B, GFAP and UCH-L1 in an aging population.Clinical chemistry and laboratory medicine · 2024Article
- Article
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8 authors.
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Abstract
Glial fibrillary acidic protein (GFAP) is a well-established astrocytic biomarker for the diagnosis, monitoring and outcome prediction of traumatic brain injury (TBI). Few studies stated an accumulation of neuronal GFAP that was observed in various brain pathologies, including traumatic brain injuries. As the neuronal immunopositivity for GFAP in Alzheimer patients was shown to cross-react with non-GFAP epitopes, the neuronal immunopositivity for GFAP in TBI patients should be challenged. In this study, cerebral and cerebellar tissues of 52 TBI fatalities and 17 controls were screened for immunopositivity for GFAP in neurons by means of immunohistochemistry and immunofluorescence. The results revealed that neuronal immunopositivity for GFAP is most likely a staining artefact as negative controls also revealed neuronal GFAP staining. However, the phenomenon was twice as frequent for TBI fatalities compared to non-TBI control cases (12 vs. 6%). Neuronal GFAP staining was observed in the pericontusional zone and the ipsilateral hippocampus, but was absent in the contralateral cortex of TBI cases. Immunopositivity for GFAP was significantly correlated with the survival time (r = 0.306, P = 0.015), but no correlations were found with age at death, sex nor the post-mortem interval in TBI fatalities. This study provides evidence that the TBI-associated neuronal immunopositivity for GFAP is indeed a staining artefact. However, an absence post-traumatic neuronal GFAP cannot readily be assumed. Regardless of the particular mechanism, this study revealed that the artefact/potential neuronal immunopositivity for GFAP is a global, rather than a regional brain phenomenon and might be useful for minimum TBI survival time determinations, if certain exclusion criteria are strictly respected.
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