ArticlePeerJ2021
The effects of genotypes and media composition on callogenesis, regeneration and cell suspension culture of chamomile (
Article in PeerJ, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 25 citations in OpenAlex.
- Establishment of a High-Efficiency In Vitro Regeneration andBiotech (Basel (Switzerland)) · 2026Article
- Machine learning optimized callogenesis in Justicia gendarussa Burm. f. and phytochemical profiling of in vitro derived callus and leaf extracts.BMC biotechnology · 2026Article
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- Efficient callus induction and regeneration of tea plant (Camellia sinensis).Scientific reports · 2025Article
- Somatic Mutation Accumulations in Micropropagated Cannabis Are Proportional to the Number of Subcultures.Plants (Basel, Switzerland) · 2024Article
- Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets ofPlants (Basel, Switzerland) · 2023Article
- Natural Compounds with Antimicrobial Properties in Cosmetics.Pathogens (Basel, Switzerland) · 2023Review
- Somaclonal Variation-Advantage or Disadvantage in Micropropagation of the Medicinal Plants.International journal of molecular sciences · 2023Review
- The Efficient and Easy Micropropagation Protocol ofPlants (Basel, Switzerland) · 2021Article
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Authors and funding
7 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChamomile is an important herb being used widely for medicinal purposes. Its multitherapeutic, cosmetic, and nutritional values have been established through years of traditional and scientific use and research. Increased use of medicinal plants necessitates rational use as well as sustainable production of such genetic resources. Plant in vitro micro-propagation poses unique opportunities for sustainable production of medicinal herbs, their regrowth and conservation. The present study aimed to investigate the effects of different explants, plant growth regulators (PGRs) combinations and media type on callogenesis, in vitro regeneration and cell suspension of six chamomile genotypes to enhance its sustainable production.
methodsThe shoot, lateral sprout, and leaf derived explants of six chamomile genotypes including Isfahan, Shiraz, Kazeron, Goral, Sharokashari and Presso were used for direct and indirect regeneration. For indirect regeneration various doses of NAA and kinetin were used to induce calli which were cultured on MS media containing PGRs for direct and indirect regeneration. Later, cell suspension was established and morphological characterization of CrO RESULTS AND DISCUSSION: Our findings revealed that the highest callus percentage and callus volume were observed from lateral sprouts and shoots of genotype Isfahan on MS medium containing 1 mg/L NAA and 1 mg/L kinetin. The in vitro regeneration was found to be genotype dependent while 77% and 77.5% was the highest percentage for indirect and direct regeneration, respectively. Additionally, the maximum shoot number (two shoots/explant) and shoot length (2.22 cm) were also observed in Isfahan genotype. Cell suspension culture showed the highest fresh weight (18.59 g) and dry weight (1.707 g) with 0.75 g inoculum of the callus derived from lateral sprouts cultured on MS medium. Microscopy of CrO
conclusionThe callogenesis, organogenesis, and cell suspension culture of chamomile may be genotype dependent. Hence, optimization of media ingredients and culture conditions is of utmost importance for devising tissue culture based conservation strategy of any chamomile genotype and secondary metabolite production.
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