ArticlePediatric research2022
Blood transcriptomic markers of necrotizing enterocolitis in preterm pigs.
Article in Pediatric research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 16 citations in OpenAlex.
- Using Gut Microbiota and Gut Metabolites as Biomarkers for Diseases in Monogastric Farm Animals.Animals : an open access journal from MDPI · 2026Review
- Neonatal enteral antibiotics reduce gut inflammation and delay systemic immune development in preterm pigs.Pediatric research · 2026Article
- Targeted immune response gene expression profiles linked with necrosis and mortality in preterm infants with surgical necrotizing enterocolitis.World journal of pediatric surgery · 2026Article
- Integrated multi-omics reveals the Bacillus amyloliquefaciens BA40 against Clostridium perfringens infection in weaned piglets.Journal of advanced research · 2025Article
- Multi-strain probiotic administration decreases necrotizing enterocolitis severity and alters the epigenetic profile in mice.Pediatric research · 2025Article
- Data-independent acquisition-based blood proteomics unveils predictive biomarkers for neonatal necrotizing enterocolitis.Analytical and bioanalytical chemistry · 2025Article
- Predictive value of biomarkers in neonatal necrotizing enterocolitis.Frontiers in pediatrics · 2025Review
- Science and Faith to Understand Milk Bioactivity for Infants.Nutrients · 2024Review
- Identifying immune signatures of sepsis to increase diagnostic accuracy in very preterm babies.Nature communications · 2024Article
- Preterm pigs for preterm birth research: reasonably feasible.Frontiers in physiology · 2023Review
- Brain lipidomics and neurodevelopmental outcomes in intrauterine growth restricted piglets fed dairy or vegetable fat diets.Scientific reports · 2022Article
- Biomarkers of gut injury in neonates - where are we in predicting necrotising enterocolitis?Frontiers in pediatrics · 2022Review
- Emerging prediction methods for early diagnosis of necrotizing enterocolitis.Frontiers in medicine · 2022Review
- Editorial: Immunity in Compromised Newborns.Frontiers in immunology · 2021Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNecrotizing enterocolitis (NEC), a severe gut disorder in preterm infants, is difficult to predict due to poor specificity and sensitivity of clinical signs and biomarkers. Using preterm piglets as a model, we hypothesized that early development of NEC affects blood gene expression, potentially related to early systemic immune responses.
methodsA retrospective analysis of clinical, tissue, and blood data was performed on 129 formula-fed piglets with NEC diagnosis at necropsy on day 5. Subgroups of NEC (n = 20) and control piglets (CON, n = 19) were analyzed for whole-blood transcriptome.
resultsPreterm piglets had variable NEC lesions, especially in the colon region, without severe clinical signs (e.g. normal growth, activity, hematology, digestion, few piglets with bloody stools). Transcriptome analysis showed 344 differentially expressed genes (DEGs) between NEC and CON piglets. Validation experiment showed that AOAH, ARG2, FKBP5, PAK2, and STAT3 were among the genes affected by severe lesions on day 5, when analyzed in whole blood and in dried blood spots (DBS).
conclusionWhole-blood gene expressions may be affected in preterm pigs before clinical signs of NEC get severe. Blood gene expression analysis, potentially using DBS samples, is a novel tool to help identify new early biomarkers of NEC. IMPACT: Preterm pig model was used to investigate if blood transcriptomics could be used to identify new early blood biomarkers of NEC progression. Whole-blood transcriptome revealed upregulation of target genes in NEC cases when clinical symptoms are subtle, and mainly colon regions were affected. Differential NEC-associated gene expressions could be detected also in dried blood spots, potentially allowing easy collection of small blood volumes in infants.
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