ArticleVeterinary research2021
In vivo and in vitro models show unexpected degrees of virulence among Toxoplasma gondii type II and III isolates from sheep.
Article in Veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- Pathogenesis of Brucella abortus and Brucella melitensis in bovine and ovine-derived trophoblasts and macrophages and impaired intracellular trafficking of the Rev1 vaccine strain.Veterinary research · 2026Article
- Development and validation of a gra1-bag1 RT-qPCR assay as an alternative to the mouse bioassay for assessing Toxoplasma gondii viability.Scientific reports · 2026Article
- The role of TgEfhc3 in Toxoplasma gondii biology and its prospects as a subunit vaccine.Parasites & vectors · 2026Article
- Elevated Liver Enzymes and Genotype I of T. gondii Among Liver Cancer Patients: Evidence of a Potential Link.Asian Pacific journal of cancer prevention : APJCP · 2026Article
- An inactivated vaccine against acquired Toxoplasma gondii infection in pigs as a tool to minimize the zoonotic transmission risk.Veterinary research · 2025Article
- Molecular detection and characterisation ofInternational journal for parasitology. Parasites and wildlife · 2025Article
- Characterization of a novel murine model of Toxoplasma gondii infection using oocysts of a recently obtained Type III isolate.Veterinary research · 2025Article
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- Treatment with BKI-1748 afterAntimicrobial agents and chemotherapy · 2025Article
- Proteome changes duringFrontiers in cellular and infection microbiology · 2025Article
- A population study of Toxoplasma gondii in the Amazon region expands current knowledge of the genetic diversity in South America.PLoS neglected tropical diseases · 2024Article
- Genotyping ofParasitology · 2024Article
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- Modeling the human placental barrier to understandFrontiers in cellular and infection microbiology · 2023Review
- Effects of Ovine Monocyte-Derived Macrophage Infection by Recently IsolatedAnimals : an open access journal from MDPI · 2022Article
- Article
- Review
- Unifying Virulence Evaluation inFrontiers in cellular and infection microbiology · 2022Review
- Archetypal type II and IIIFrontiers in immunology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
Toxoplasma gondii is an important zoonotic agent with high genetic diversity, complex epidemiology, and variable clinical outcomes in animals and humans. In veterinary medicine, this apicomplexan parasite is considered one of the main infectious agents responsible for reproductive failure in small ruminants worldwide. The aim of this study was to phenotypically characterize 10 Spanish T. gondii isolates recently obtained from sheep in a normalized mouse model and in an ovine trophoblast cell line (AH-1) as infection target cells. The panel of isolates met selection criteria regarding such parameters as genetic diversity [types II (ToxoDB #1 and #3) and III (#2)], geographical location, and sample of origin (aborted foetal brain tissues or adult sheep myocardium). Evaluations of in vivo mortality, morbidity, parasite burden and histopathology were performed. Important variations between isolates were observed, although all isolates were classified as "nonvirulent" (< 30% cumulative mortality). The isolates TgShSp16 (#3) and TgShSp24 (#2) presented higher degrees of virulence. Significant differences were found in terms of in vitro invasion rates and tachyzoite yield at 72 h post-inoculation (hpi) between TgShSp1 and TgShSp24 isolates, which exhibited the lowest and highest rates, respectively. The study of the CS3, ROP18 and ROP5 loci allelic profiles revealed only type III alleles in ToxoDB #2 isolates and type II alleles in the #1 and #3 isolates included. We concluded that there are relevant intra- and inter-genotype virulence differences in Spanish T. gondii isolates, which could not be inferred by genetic characterization using currently described molecular markers.
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