ArticlePLoS genetics2021
Disrupting hierarchical control of nitrogen fixation enables carbon-dependent regulation of ammonia excretion in soil diazotrophs.
Article in PLoS genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Engineered diazotrophs with host-inducible nitrogen supply systems: Transforming rice farming through innovative nitrogen biofertilizers.Journal of integrative plant biology · 2026Review
- Sentinel plants enable quantitative monitoring of bioavailable nitrate in soils and microbial environments.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Structural analysis of the NifL-NifA complex reveals the molecular basis of anti-activation of nitrogen fixation gene expression in Azotobacter vinelandii.The FEBS journal · 2026Article
- Article
- Enhanced Urea Production in the Diazotroph Azotobacter vinelandii as a Means of Stable Nitrogen Biofertiliser Production.Microbial biotechnology · 2025Article
- Article
- Engineering nitrogen-fixing microbiomes with waste-derived carbon sources: toward circular and resilient biofertilizer solution.Frontiers in microbiology · 2025Article
- Precision control of ammonium release in Azotobacter vinelandii.Microbial biotechnology · 2024Article
- Competitive fitness and stability of ammonium-excreting Azotobacter vinelandii strains in the soil.Applied microbiology and biotechnology · 2024Article
- Scripting a new dialogue between diazotrophs and crops.Trends in microbiology · 2024Review
- Enhanced extracellular ammonium release in the plant endophyteMicrobiology spectrum · 2024Article
- Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity.Microbial cell factories · 2023Article
- Rhizopine biosensors for plant-dependent control of bacterial gene expression.Environmental microbiology · 2023Article
- Molecular Mechanism and Agricultural Application of the NifA-NifL System for Nitrogen Fixation.International journal of molecular sciences · 2023Review
- How can we possibly resolve the planet's nitrogen dilemma?Microbial biotechnology · 2023Article
- Interactions between paralogous bacterial enhancer-binding proteins enable metal-dependent regulation of alternative nitrogenases in Azotobacter vinelandii.Molecular microbiology · 2022Article
- Control of nitrogen fixation and ammonia excretion in Azorhizobium caulinodans.PLoS genetics · 2022Article
- Engineered plant control of associative nitrogen fixation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Genetic Determinants of Ammonium Excretion inApplied and environmental microbiology · 2022Article
- Improved Stability of Engineered Ammonia Production in the Plant-SymbiontACS synthetic biology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The energetic requirements for biological nitrogen fixation necessitate stringent regulation of this process in response to diverse environmental constraints. To ensure that the nitrogen fixation machinery is expressed only under appropriate physiological conditions, the dedicated NifL-NifA regulatory system, prevalent in Proteobacteria, plays a crucial role in integrating signals of the oxygen, carbon and nitrogen status to control transcription of nitrogen fixation (nif) genes. Greater understanding of the intricate molecular mechanisms driving transcriptional control of nif genes may provide a blueprint for engineering diazotrophs that associate with cereals. In this study, we investigated the properties of a single amino acid substitution in NifA, (NifA-E356K) which disrupts the hierarchy of nif regulation in response to carbon and nitrogen status in Azotobacter vinelandii. The NifA-E356K substitution enabled overexpression of nitrogenase in the presence of excess fixed nitrogen and release of ammonia outside the cell. However, both of these properties were conditional upon the nature of the carbon source. Our studies reveal that the uncoupling of nitrogen fixation from its assimilation is likely to result from feedback regulation of glutamine synthetase, allowing surplus fixed nitrogen to be excreted. Reciprocal substitutions in NifA from other Proteobacteria yielded similar properties to the A. vinelandii counterpart, suggesting that this variant protein may facilitate engineering of carbon source-dependent ammonia excretion amongst diverse members of this family.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.