ArticlePloS one2021
A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- A Label-Free Cell-Based Biosensor Method for Ethanol Quantification Using Temperature-Induced Spontaneous Cell Detachment.Biosensors · 2026Article
- Recent Knowledge in the Application ofAntibiotics (Basel, Switzerland) · 2025Review
- Flow cytometric procedures for deep characterization of nanoparticles.Biology methods & protocols · 2025Article
- Extracellular Self-DNA Effects on Yeast Cell Cycle and Transcriptome during Batch Growth.Biomolecules · 2024Article
- Extracellular DNA secreted in yeast cultures is metabolism-specific and inhibits cell proliferation.Microbial cell (Graz, Austria) · 2023Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.
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