ArticleBiotechnology and bioengineering2021
Travelling ultrasound promotes vasculogenesis of three-dimensional-monocultured human umbilical vein endothelial cells.
Article in Biotechnology and bioengineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- ID3-directed sonogenetics to enhance osteogenesis and bone defect healing.International journal of oral science · 2026Article
- Acoustic technologies for the orchestration of cellular functions for therapeutic applications.Science advances · 2025Review
- Neuromodulation with Ultrasound: Hypotheses on the Directionality of Effects and Community Resource.medRxiv : the preprint server for health sciences · 2025Article
- Influence of Exposure Time and Driving Frequency on Cytotoxicity in In Vitro Ultrasound With Constant Mechanical Indices.Engineering in life sciences · 2025Article
- Mechanical strategies to promote vascularization for tissue engineering and regenerative medicine.Burns & trauma · 2024Review
- Low-Intensity Ultrasound Reduces Brain Infarct Size by Upregulating Phosphorylated Endothelial Nitric Oxide in Mouse Model of Middle Cerebral Artery Occlusion.Ultrasound in medicine & biology · 2023Article
- Titanium Culture Vessel Presenting Temperature Gradation for the Thermotolerance Estimation of Cells.Cyborg and bionic systems (Washington, D.C.) · 2023Article
- Transcranial ultrasound stimulation applied in ischemic stroke rehabilitation: A review.Frontiers in neuroscience · 2022Review
- Development of accurate temperature regulation culture system with metallic culture vessel demonstrates different thermal cytotoxicity in cancer and normal cells.Scientific reports · 2021Article
- Travelling ultrasound promotes vasculogenesis of three-dimensional-monocultured human umbilical vein endothelial cells.Biotechnology and bioengineering · 2021Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To generate three-dimensional tissue in vitro, promoting vasculogenesis in cell aggregates is an important factor. Here, we found that ultrasound promoted vasculogenesis of human umbilical vein endothelial cells (HUVECs). Promotion of HUVEC network formation and lumen formation were observed using our method. In addition to morphological evaluations, protein expression was quantified by western blot assays. As a result, expression of proteins related to vasculogenesis and the response to mechanical stress on cells was enhanced by exposure to ultrasound. Although several previous studies have shown that ultrasound may promote vasculogenesis, the effect of ultrasound was unclear because of unregulated ultrasound, the complex culture environment, or two-dimensional-cultured HUVECs that cannot form a lumen structure. In this study, regulated ultrasound was propagated on three-dimensional-monocultured HUVECs, which clarified the effect of ultrasound on vasculogenesis. We believe this finding may be an innovation in the tissue engineering field.
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