Evidence map›Paper›PMID 34109827›Full record

ArticleCartilage2021

Hypertonic Dextrose Stimulates Chondrogenic Cells to Deposit Collagen and Proliferate.

Elisha Johnston, Yi Kou, Jason Junge, Lin Chen, Andrew Kochan, Michael Johnston, David Rabago

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cartilage, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 12 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Cartilage · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Article
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  9. Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Elisha JohnstonUniversity of California Los Angeles, Los Angeles, CA, USA.
Yi KouMolecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, CA, USA.
Jason JungeImaging Services, Advanced Light Microscopy Core, Translational Imaging Center, University of Southern California, Los Angeles, CA, USA.
Lin ChenDepartment of Chemistry and Biological Sciences, University of Southern California, Los Angeles, CA, USA.
Andrew KochanHealing Arts Research, Sherman Oaks, CA, USA.
Michael JohnstonIndependent Researcher, Rancho Palos Verdes, CA, USA.
David RabagoDepartment of Family and Community Medicine, Penn State College of Medicine, Hershey, PA, USA.ORCID 0000-0002-1347-3603
University of Southern California · USMarymount California University · USPenn State Milton S. Hershey Medical Center · USUniversity of California, Los Angeles · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveHypertonic dextrose (HD) injections (prolotherapy) for osteoarthritis are reported to reduce pain. Cartilage regeneration is hypothesized as a mechanism. This

designATDC5 chondrogenic cells were cultured in normoglycemic DMEM/F12 medium, treated with concentrations of HD (4-400 mM), and assessed with PrestoBlue. Advanced light microscopy was used to conduct live imaging of collagen deposition through second harmonic generation microscopy (SHG) and proliferation via 2-photon excitation microscopy. Proliferation was additionally assessed with hemocytometer counts.

resultsA linear regression model found that, relative to the 4 mM baseline control, cells treated with 200 mM had a higher mean absorbance (

conclusionsA 250 mM HD solution appears to be associated with increased metabolic activity of chondrocytes, increased collagen deposition, and increased chondrocyte proliferation. These results support clinical prolotherapy research suggesting that intra-articular HD joint injections reduce knee pain. Further study of HD and cellular processes is warranted.

Indexed as

Osteoarthritis, KneeProlotherapyChondrogenesisCollagenGlucoseHumansCollagenGlucosearticular cartilagecellschondrocytescollagenpreclinical researchprolotherapytissue

Identifiers

PMID34109827
PMCPMC8804764
OpenAlexW3170239805

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.