Evidence map›Paper›PMID 34109572›Full record

ArticlePharmacological reports : PR2021

Modulation of DNA damage response by targeting ATM kinase using newly synthesized di-phenoxy acetamide (DPA) analogs to induce anti-neoplasia.

Fares Hezam Al-Ostoot, Ankith Sherapura, Vikas H Malojirao, Prabhu Thirusangu, Tahani I Al-Muhimeed, Shaukath Ara Khanum, B T Prabhakar

Abstract read
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Article in Pharmacological reports : PR, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 4 countries.

Fares Hezam Al-OstootDepartment of Chemistry, Yuvaraja's College, University of Mysore, Mysuru, India.
Ankith SherapuraMolecular Biomedicine Laboratory, Postgraduate Department of Studies and Research in Biotechnology, Sahyadri Science College, Kuvempu University, Shivamogga, Karnataka, India.
Vikas H MalojiraoMolecular Biomedicine Laboratory, Postgraduate Department of Studies and Research in Biotechnology, Sahyadri Science College, Kuvempu University, Shivamogga, Karnataka, India.
Prabhu ThirusanguMolecular Biomedicine Laboratory, Postgraduate Department of Studies and Research in Biotechnology, Sahyadri Science College, Kuvempu University, Shivamogga, Karnataka, India.
Tahani I Al-MuhimeedDepartment of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Shaukath Ara Khanum *Department of Chemistry, Yuvaraja's College, University of Mysore, Mysuru, India. shaukathah@ycm.uni-mysore.ac.in.ORCID http://orcid.org/0000-0003-4571-6419
B T Prabhakar *Molecular Biomedicine Laboratory, Postgraduate Department of Studies and Research in Biotechnology, Sahyadri Science College, Kuvempu University, Shivamogga, Karnataka, India. prabhakarbt1@kussc.org.
Kuvempu University · INUniversity of Mysore · INHouston Methodist · USKing Saud University · SAMayo Clinic · US

Funding

SERB SERB [EMR/2017/00088/dated 3/01/2019]VGST [Project sanction order: No.VGST/CISEE/282/2012-13]VGST VGST(VGST/P-2/CISEE/GRD-231/2013-14)
6 · The paper itself

Abstract

backgroundImbalance and instability in the structure of the DNA have become major characteristics of cancer. In response to DNA damage, DNA damage response (DDR) protein, ataxia telangiectasia mutated (ATM), plays a pivotal role in the modulation of regulatory regions responsible for inhibition of apoptosis, thereby neoplastic progression.

methodsA new series of DPA (7a-t) were synthesized, characterized. Anti-proliferative studies to identify the lead compound were carried out by LDH and MTT assay. Apoptosis/DNA damage was measured through FACS, Annexin-v staining, TUNEL and Comet assay. Elucidation of molecular mechanism through immunoblot and further validation of the drug effect through in vivo approaches.

resultsInitial in vitro anti-proliferative screening of Compounds DPA (7a-t) against multiple cancer cell lines identified Compound DPA (7n) as a potent cytotoxic molecule with IC

conclusionsCompound DPA (7n) emerged as a potent proapoptotic and anti-neoplastic agent by inhibiting ATM kinase activity both in vitro and in vivo. The conferring results ascertain that the drug could be developed as a new ATM kinase inhibitor with anti-cancer capacity.

Indexed as

DNA DamageDrug Delivery SystemsAcetamidesAnimalsAntineoplastic AgentsApoptosisCell Line, TumorDNA RepairHumansMiceMice, Inbred BALB CMolecular StructureNeoplasms, ExperimentalXenograft Model Antitumor AssaysAcetamidesAntineoplastic AgentsAnti-neoplasiaApoptosisATM kinaseDDRDi-phenoxy acetamide analogs

Identifiers

PMID34109572
OpenAlexW3173017253

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.