Evidence map›Paper›PMID 34108852›Full record

ArticleAdvanced functional materials2019

3D Printed Cartilage-Like Tissue Constructs with Spatially Controlled Mechanical Properties.

Bruna A G de Melo, Yasamin A Jodat, Shreya Mehrotra, Michelle A Calabrese, Tom Kamperman, Biman B Mandal, Maria H A Santana, Eben Alsberg, Jeroen Leijten, Su Ryon Shin

Abstract read
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In one paragraph

Article in Advanced functional materials, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.

0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

51 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Bruna A G de MeloDivision of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Cambridge, MA 02139, USA.
Yasamin A JodatDivision of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Cambridge, MA 02139, USA.
Shreya MehrotraDivision of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Cambridge, MA 02139, USA.
Michelle A CalabreseDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Tom KampermanDepartment of Developmental BioEngineering, University of Twente, Enschede, Overijssel 7522 NB, The Netherlands.
Biman B MandalDepartment of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, India.
Maria H A SantanaDepartment of Engineering of Materials and Bioprocesses School of Chemical Engineering, University of Campinas, Campinas, SP 13083-852, Brazil.
Eben AlsbergDepartments of Bioengineering and Orthopaedics, University of Illinois, Chicago, IL 60607, USA.
Jeroen Leijten *Department of Developmental BioEngineering, University of Twente, Enschede, Overijssel 7522 NB, The Netherlands.
Su Ryon Shin *Division of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Cambridge, MA 02139, USA.

Funding

Oxygen generating bioinks for 3D printed bone implantsR01AR074234 · NIAMS · BRIGHAM AND WOMEN'S HOSPITAL · PI SHIN, SU RYON · 2018 to 2022
$1.9M
Programmable multimaterial bioprinting of 3D vascularized tissue constructsR21EB026824 · NIBIB · BRIGHAM AND WOMEN'S HOSPITAL · PI SHIN, SU RYON · 2018 to 2020
$685k
NIAMS NIH HHS R01 AR074234NIBIB NIH HHS R21 EB026824
6 · The paper itself

Abstract

Developing biomimetic cartilaginous tissues that support locomotion while maintaining chondrogenic behavior is a major challenge in the tissue engineering field. Specifically, while locomotive forces demand tissues with strong mechanical properties, chondrogenesis requires a soft microenvironment. To address this challenge, 3D cartilage-like tissue is bioprinted using two biomaterials with different mechanical properties: a hard biomaterial to reflect the macromechanical properties of native cartilage, and a soft biomaterial to create a chondrogenic microenvironment. To this end, a hard biomaterial (MPa order compressive modulus) composed of an interpenetrating polymer network (IPN) of polyethylene glycol (PEG) and alginate hydrogel is developed as an extracellular matrix (ECM) with self-healing properties, but low diffusive capacity. Within this bath supplemented with thrombin, fibrinogen containing human mesenchymal stem cell (hMSC) spheroids is bioprinted forming fibrin, as the soft biomaterial (kPa order compressive modulus) to simulate cartilage's pericellular matrix and allow a fast diffusion of nutrients. The bioprinted hMSC spheroids improve viability and chondrogenic-like behavior without adversely affecting the macromechanical properties of the tissue. Therefore, the ability to print locally soft and cell stimulating microenvironments inside of a mechanically robust hydrogel is demonstrated, thereby uncoupling the micro- and macromechanical properties of the 3D printed tissues such as cartilage.

Indexed as

bioprintingcartilagefibrinIPNspheroids

Identifiers

PMID34108852
PMCPMC8186324

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.