ArticleNature protocols2021
Implantation of Neuropixels probes for chronic recording of neuronal activity in freely behaving mice and rats.
Article in Nature protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 84 citations in OpenAlex.
- Zwitterionic Polymer Coating Enabled Chronic Dopamine Sensing and Electrophysiology Recording in Free-Moving Mice.Advanced healthcare materials · 2026Article
- SCREWx: A Screwless, Chronic, Recoverable, and Lightweight Neuropixels fixture for freely-moving rodents.bioRxiv : the preprint server for biology · 2026Article
- Experience shapes the transformation of olfactory representations along the cortico-hippocampal pathway.eLife · 2026Article
- Large-scale electrophysiology at single-spike resolution.Nature reviews. Neuroscience · 2026Review
- Top-down regulation of ingestive behavior fragmentation.bioRxiv : the preprint server for biology · 2026Article
- Zwitterionic polymer coating enabled chronic dopamine sensing and electrophysiology recording in free-moving mice.bioRxiv : the preprint server for biology · 2026Article
- Accumbal calcium-permeable AMPA receptors orchestrate neuronal ensembles underlying social attachment.Nature communications · 2026Article
- Protocol for testing global neuronal workspace and integrated information theories of consciousness in non-human primates and mice.PloS one · 2026Article
- A Translational Platform for Polyimide Neural Interfaces: Polyimide Synthesis and in Vivo Evaluation in Epileptic Mice.IEEE access : practical innovations, open solutions · 2026Article
- A modular, adaptable, and accessible implant kit for chronic electrophysiological recordings in rats.Cell reports methods · 2025Article
- Phase synchrony between prefrontal noradrenergic and cholinergic signals indexes inhibitory control.Nature communications · 2025Article
- Article
- Sleep need-dependent plasticity of a thalamic circuit promotes homeostatic recovery sleep.Science (New York, N.Y.) · 2025Article
- Automatic OptoDrive for Extracellular Recordings and Optogenetic Stimulation in Freely Moving Mice.eNeuro · 2025Article
- Article
- Tracking neurons across days with high-density probes.Nature methods · 2025Article
- Article
- The serotonergic psychedelic DOI impairs deviance detection in the auditory cortex.Journal of neurophysiology · 2025Article
- NeuroCarto: A Toolkit for Building Custom Read-out Channel Maps for High Electrode-count Neural Probes.Neuroinformatics · 2025Article
- Functional connectivity drifts during sleep as a marker of fluctuations in the level of consciousness.Neuroscience of consciousness · 2025Article
Corrections and comments
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Authors and funding
7 authors at 6 institutions in 2 countries.
Funding
Abstract
How dynamic activity in neural circuits gives rise to behavior is a major area of interest in neuroscience. A key experimental approach for addressing this question involves measuring extracellular neuronal activity in awake, behaving animals. Recently developed Neuropixels probes have provided a step change in recording neural activity in large tissue volumes with high spatiotemporal resolution. This protocol describes the chronic implantation of Neuropixels probes in mice and rats using compact and reusable 3D-printed fixtures. The fixtures facilitate stable chronic in vivo recordings in freely behaving rats and mice. They consist of two parts: a covered main body and a skull connector. Single-, dual- and movable-probe fixture variants are available. After completing an experiment, probes are safely recovered for reimplantation by a dedicated retrieval mechanism. Fixture assembly and surgical implantation typically take 4-5 h, and probe retrieval takes ~30 min, followed by 12 h of incubation in probe cleaning agent. The duration of data acquisition depends on the type of behavioral experiment. Since our protocol enables stable, chronic recordings over weeks, it enables longitudinal large-scale single-unit data to be routinely obtained in a cost-efficient manner, which will facilitate many studies in systems neuroscience.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.