ArticleScientific reports2021
DNA degradation in human teeth exposed to thermal stress.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- Pooled it
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- Plate-Based High-Throughput Fluorescence Assay for Assessing Enveloped Virus Integrity.Biomacromolecules · 2024Article
- Forensic Microbiology: When, Where and How.Microorganisms · 2024Review
- Quantitative Analysis of Genomic DNA Degradation ofGels (Basel, Switzerland) · 2024Article
- Native microbiome dominates over host factors in shaping the probiotic genetic evolution in the gut.NPJ biofilms and microbiomes · 2023Article
- Identification of the Remains of an Adult Using DNA from Their Deciduous Teeth as a Reference Sample.Medicina (Kaunas, Lithuania) · 2023Article
- The intervertebral discs' fibrocartilage as a DNA source for genetic identification in severely charred cadavers.Forensic science, medicine, and pathology · 2022Article
- Dental DNA as an Indicator of Post-Mortem Interval (PMI): A Pilot Research.International journal of molecular sciences · 2022Article
- The Perfect Match: Assessment of Sample Collection Efficiency for Immunological and Molecular Findings in Different Types of Fabrics.International journal of molecular sciences · 2022Article
- Forensic DNA analysis of heat-exposed unrestored teeth: Temperature and duration effects.Journal of oral and maxillofacial pathology : JOMFPArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human identification from burned remains poses a challenge to forensic laboratories, and DNA profiling is widely used for this purpose. Our aim was to evaluate the effect of temperature on DNA degradation in human teeth. Thirty teeth were exposed to temperatures of 100, 200, or 400 °C for 60 min. DNA was quantified by Real-Time qPCR (Quantifiler Human DNA Quantification Kit) and fluorescence spectroscopy (Qubit 3.0 Fluorometer). DNA degradation was evaluated by using STR markers (AmpFLSTR Identifiler Plus PCR Amplification Kit) to determine the allele and locus dropout, inter-locus balance, and degradation slope (observed (Oa) to expected (Ea) locus peak height ratio against the molecular weight). Most of the genomic DNA was degraded between 100 °C and 200 °C. At 100 °C, locus dropout ratios showed significant differences between the largest loci (FGA, D7S820, D18S51, D16S539, D2S1338 and CSF1PO) and amelogenin. Inter-locus balance values significantly differed between all dye channels except between NED and PET. The dropout ratio between D18S51 (NED) and amelogenin (PET) can be recommended for the evaluation of DNA degradation. The Oa/Ea regression model can predict locus peak heights in DNA degradation (R
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