ArticleScientific reports2021
Development of loop-mediated isothermal amplification (LAMP) assay using SYBR safe and gold-nanoparticle probe for detection of Leishmania in HIV patients.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Development of a species-specific PCR-lateral flow assay integrated with SYBR Safe for rapid authentication of commercial Polygoni Multiflori Caulis (Ye-Jiao-Teng).Journal of natural medicines · 2026Article
- Miltefosine and emerging Leishmania (Mundinia) in Southeast Asia: Molecular insights, therapeutic challenges, and future strategic implementation.PLoS neglected tropical diseases · 2026Review
- LAMP-coupled CRISPR-Cas12a assays: A promising new tool for molecular diagnosis of leishmaniasis.PLoS neglected tropical diseases · 2026Article
- Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection ofJournal of nematology · 2026Article
- Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia.Frontiers in microbiology · 2026Article
- LAMP using distance based paper device for quantitative detection of ESBL producing Escherichia coli in UTI patients.Scientific reports · 2025Article
- Rapid and Cost-EffectiveDiagnostics (Basel, Switzerland) · 2025Article
- Development of a loop-mediated isothermal amplification assay for the rapid detection of Alongshan virus.The Journal of general virology · 2025Article
- Development of colorimetric and fluorescent closed tube LAMP assay using simplified extraction for diagnosis of Meloidogyne enterolobii in root tissues.Scientific reports · 2025Article
- Loop-mediated isothermal amplification assay for rapid diagnosis soybean damping-off disease caused byFrontiers in cellular and infection microbiology · 2025Article
- Platinum Group Metals against Parasites: State of the Art and Future Perspectives.Medicinal chemistry (Shariqah (United Arab Emirates)) · 2025Review
- A colorimetric loop-mediated isothermal amplification assay (c-LAMP) for rapid detection ofHeliyon · 2024Article
- Loop-Mediated Isothermal Amplification Assay Using Gold Nanoparticles for DetectingDiagnostics (Basel, Switzerland) · 2024Article
- Comparison of the Diagnostic Performances of Five Different Tests in Diagnosing Visceral Leishmaniasis in an Endemic Region of Ethiopia.Diagnostics (Basel, Switzerland) · 2024Article
- Article
- Genetic variation and geographic distribution of Leishmania orientalis and Leishmania martiniquensis among Leishmania/HIV co-infection in Thailand.Scientific reports · 2023Article
- Article
- Validation of quantitative loop-mediated isothermal amplification assay using a fluorescent distance-based paper device for detection of Escherichia coli in urine.Scientific reports · 2023Article
- Distance-based paper device using combined SYBR safe and gold nanoparticle probe LAMP assay to detect Leishmania among patients with HIV.Scientific reports · 2022Article
- Isothermal Nucleic Acid Amplification to Detect Infection Caused by Parasites of the Trypanosomatidae Family: A Literature Review and Opinion on the Laboratory to Field Applicability.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Asymptomatic leishmaniasis cases have continuously increased, especially among patients with HIV who are at risk to develop further symptoms of cutaneous and visceral leishmaniasis. Thus, early diagnosis using a simple, sensitive and reliable diagnostic assay is important because populations at risk mostly reside in rural communities where laboratory equipment is limited. In this study, the highly sensitive and selective determination of Leishmania infection in asymptomatic HIV patients was achieved using dual indicators (SYBR safe and gold-nanoparticle probe; AuNP-probe) in one-step LAMP method based on basic instruments. The assay can be simply evaluated under the naked eye due to clear interpretation of fluorescent emission of LAMP-SYBR safe dye-complex and colorimetric precipitate of specific AuNP-probes. The sensitivities and specificities of fluorescent SYBR safe dye and AuNP-probe indicators were equal, which were as high as 94.1 and 97.1%, respectively. Additionally, detection limits were 10
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