ArticleBMC biology2021
The Wnt-specific astacin proteinase HAS-7 restricts head organizer formation in Hydra.
Article in BMC biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Mutual inhibition model of pattern formation: The role of Wnt-Dickkopf interactions in driving Hydra body axis formation.PLoS computational biology · 2026Article
- Hydra at the Crossroads of Evolution: Insights into Cell-type Diversification, Regeneration, and Epigenetic Regulation.Results and problems in cell differentiation · 2026Review
- A transphyletic study of metazoan β-catenin protein complexes.Zoological letters · 2024Article
- Genomic and single-cell analyses reveal genetic signatures of swimming pattern and diapause strategy in jellyfish.Nature communications · 2024Article
- Mechanical characterization of regenerating Hydra tissue spheres.Biophysical journal · 2024Article
- TheBiomedicines · 2024Article
- Article
- Wnt/β-catenin signaling induces axial elasticity patterns ofiScience · 2023Article
- Article
- Structural and evolutionary insights into astacin metallopeptidases.Frontiers in molecular biosciences · 2022Article
- Cellular, Metabolic, and Developmental Dimensions of Whole-Body Regeneration inCold Spring Harbor perspectives in biology · 2021Review
- The Aquatic InvertebrateFrontiers in cell and developmental biology · 2021Article
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Authors and funding
15 authors at 6 institutions in 4 countries.
Funding
Abstract
backgroundThe Hydra head organizer acts as a signaling center that initiates and maintains the primary body axis in steady state polyps and during budding or regeneration. Wnt/beta-Catenin signaling functions as a primary cue controlling this process, but how Wnt ligand activity is locally restricted at the protein level is poorly understood. Here we report a proteomic analysis of Hydra head tissue leading to the identification of an astacin family proteinase as a Wnt processing factor.
resultsHydra astacin-7 (HAS-7) is expressed from gland cells as an apical-distal gradient in the body column, peaking close beneath the tentacle zone. HAS-7 siRNA knockdown abrogates HyWnt3 proteolysis in the head tissue and induces a robust double axis phenotype, which is rescued by simultaneous HyWnt3 knockdown. Accordingly, double axes are also observed in conditions of increased Wnt activity as in transgenic actin::HyWnt3 and HyDkk1/2/4 siRNA treated animals. HyWnt3-induced double axes in Xenopus embryos could be rescued by coinjection of HAS-7 mRNA. Mathematical modelling combined with experimental promotor analysis indicate an indirect regulation of HAS-7 by beta-Catenin, expanding the classical Turing-type activator-inhibitor model.
conclusionsWe show the astacin family protease HAS-7 maintains a single head organizer through proteolysis of HyWnt3. Our data suggest a negative regulatory function of Wnt processing astacin proteinases in the global patterning of the oral-aboral axis in Hydra.
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