ArticleThe Histochemical journal1988
Detection of peroxisomes in human liver and kidney fixed with formalin and embedded in paraffin: the use of catalase and lipid beta-oxidation enzymes as immunocytochemical markers.
Article in The Histochemical journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A brief history of the human liver peroxisome.Histochemistry and cell biology · 2026Review
- Circulating biomarkers of kidney angiomyolipoma and cysts in tuberous sclerosis complex patients.iScience · 2024Article
- Oxidative stress and the role of redox signalling in chronic kidney disease.Nature reviews. Nephrology · 2024Review
- Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium.Archives of toxicology · 2021Article
- Peroxisomes and Kidney Injury.Antioxidants & redox signaling · 2016Review
- The biogenesis protein PEX14 is an optimal marker for the identification and localization of peroxisomes in different cell types, tissues, and species in morphological studies.Histochemistry and cell biology · 2013Article
- Contributions of the immunogold technique to investigation of the biology of peroxisomes.Histochemistry and cell biology · 1996Review
- In situ heterogeneity of peroxisomal oxidase activities: an update.The Histochemical journal · 1996Review
- The importance of tissue fixation for light microscopic immunohistochemical localization of peroxisomal proteins: the superiority of Carnoy's fixative over Baker's formalin and Bouin's solution.Histochemistry and cell biology · 1995Article
- Immunocytochemical localization of peroxisomal proteins in human liver and kidney.Journal of inherited metabolic disease · 1995Article
- Secondary alterations of human hepatocellular peroxisomes.Journal of inherited metabolic disease · 1995Review
- Peroxisomal localization of the immunoreactive beta-oxidation enzymes in a neonate with a beta-oxidation defect. Pathological observations in liver, adrenal cortex and kidney.Virchows Archiv. A, Pathological anatomy and histopathology · 1991Article
- Post-mortem visualization of peroxisomes in rat and in human liver.The Histochemical journal · 1990Article
- Immunocytochemical detection of peroxisomal beta-oxidation enzymes in cryostat and paraffin sections of human post mortem liver.The Histochemical journal · 1990Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
We describe the immunocytochemical localization of four peroxisomal enzymes by light microscopy in human liver and kidney processed routinely by formalin fixation and paraffin embedding. Monospecific antisera against catalase and three enzymes of peroxisomal lipid beta-oxidation (acyl-CoA oxidase, bifunctional protein (enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase) and 3-ketoacyl-CoA thiolase) were used in conjunction with either the indirect immunoperoxidase method or the protein A-gold technique followed by silver intensification. The sections of formalin-fixed paraffin-embedded tissue had to be deparaffinized and subjected to controlled proteolysis in order to obtain satisfactory immunostaining. Under the conditions employed, peroxisomes were distinctly visualized in liver parenchymal cells with no reaction in bile duct epithelial or sinusoidal lining cells. In the kidney, peroxisomes were confined to the proximal tubular epithelial cells with negative staining of glomeruli, distal tubules and collecting ducts. A positive immunocytochemical reaction was obtained even in paraffin blocks stored for several years. The method offers a simple approach for detection of peroxisomes and evaluation of their various enzyme proteins in material processed routinely in histopathology laboratories and should prove useful in the investigation of the role of peroxisomes in human pathology for both prospective and retrospective studies.
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