ArticlePLoS neglected tropical diseases2021
An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.
Article in PLoS neglected tropical diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Prevalence of tick-borne pathogens in ixodid ticks collected from domestic animals in southeast Iran.New microbes and new infections · 2026Article
- Molecular detection and phylogenetic characterization of pathogenic and endosymbiont microorganisms in Hyalomma ticks collected from livestock.Parasites & vectors · 2026Article
- Microbiome variation between male and female Hyalomma dromedarii ticks from camels in the UAE.Scientific reports · 2025Article
- Molecular and Clinical Characterization of Crimean-Congo Hemorrhagic Fever in Bulgaria, 2015-2024.Pathogens (Basel, Switzerland) · 2025Article
- The complete mitogenome of Hyalomma anatolicum: novel insights into the phylogenetics of Hyalomma.Parasitology research · 2025Article
- A systematic review of the microbiome ofVeterinary world · 2025Review
- Article
- Differential impact of spotted fever groupFrontiers in microbiology · 2025Article
- Microbiome ofInsects · 2024Article
- Comparative analysis of the microbiota of sand fly vectors of Leishmania major and L. tropica in a mixed focus of cutaneous leishmaniasis in southeast Tunisia; ecotype shapes the bacterial community structure.PLoS neglected tropical diseases · 2024Article
- Modeling the Distribution of Dominant Hard Ticks in Southeastern Coastal Areas of the Caspian Sea.Journal of arthropod-borne diseases · 2024Article
- A comparative study of the microbiomes of the ticks Rhipicephalus microplus and Hyalomma anatolicum.Parasite (Paris, France) · 2024Article
- Microbiota profile in organs of the horseflies (Diptera: Tabanidae) in Northeastern China.Frontiers in microbiology · 2024Article
- Metagenomics Characterization ofJournal of arthropod-borne diseases · 2023Article
- Study on Hard and Soft Ticks of Domestic and Wild Animals in Western Iran.Journal of arthropod-borne diseases · 2022Article
- The Impact of RNA Interference in Tick Research.Pathogens (Basel, Switzerland) · 2022Article
- Characterization and manipulation of the bacterial community in the midgut of Ixodes ricinus.Parasites & vectors · 2022Article
- Comparative analysis of the gut microbiota of sand fly vectors of zoonotic visceral leishmaniasis (ZVL) in Iran; host-environment interplay shapes diversity.PLoS neglected tropical diseases · 2022Article
- Identification of Intestinal Fungal Microflora and Bacterial Pathogens in the Collected AdultJournal of arthropod-borne diseases · 2022Article
- Microbial composition in Hyalomma anatolicum collected from livestock in the United Arab Emirates using next-generation sequencing.Parasites & vectors · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microbial flora associated with Hyalomma anatolicum ticks was investigated using culture-dependent (CD) and independent (next generation sequencing, NGS) methods. The bacterial profiles of different organs, development stages, sexes, and of host cattle skins were analyzed using the CD method. The egg and female gut microbiota were investigated using NGS. Fourteen distinct bacterial strains were identified using the CD method, of which Bacillus subtilis predominated in eggs, larval guts and in adult female and male guts, suggesting probable transovarial transmission. Bacillus velezensis and B. subtilis were identified in cattle skin and tick samples, suggesting that skin is the origin of tick bacteria. H.anatolicum males harbour lower bacterial diversity and composition than females. The NGS analysis revealed five different bacterial phyla across all samples, Proteobacteria contributing to >95% of the bacteria. In all, 56611sequences were generated representing 6,023 OTUs per female gut and 421 OTUs per egg. Francisellaceae family and Francisella make up the vast majority of the OTUs. Our findings are consistent with interference between Francisella and Rickettsia. The CD method identified bacteria, such B. subtilis that are candidates for vector control intervention approaches such paratransgenesis whereas NGS revealed high Francisella spp. prevalence, indicating that integrated methods are more accurate to characterize microbial community and diversity.
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