Evidence map›Paper›PMID 34105582›Full record

ArticleOrganic & biomolecular chemistry2021

A promiscuous glycosyltransferase generates poly-β-1,4-glucan derivatives that facilitate mass spectrometry-based detection of cellulolytic enzymes.

Gregory S Bulmer, Ashley P Mattey, Fabio Parmeggiani, Ryan Williams, Helene Ledru, Andrea Marchesi, Lisa S Seibt, Peter Both, Kun Huang, M Carmen Galan and 3 more

Abstract read
In one paragraph

Article in Organic & biomolecular chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Gregory S BulmerManchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk.ORCID 0000-0003-4794-2858
Ashley P MatteyManchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk.ORCID 0000-0002-6564-7150
Fabio ParmeggianiManchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk and Department of Chemistry, Materials and Chemical Engineering "G. Natta", Politecnico di Milano, Milano, Italy.
Ryan WilliamsSchool of Chemistry, University of Bristol, Bristol, UK.
Helene LedruSchool of Chemistry, University of Bristol, Bristol, UK.
Andrea MarchesiManchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk.ORCID 0000-0002-1560-5921
Lisa S SeibtManchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk.ORCID 0000-0002-8230-5371
Peter BothManchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk.
Kun HuangManchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk.
M Carmen GalanSchool of Chemistry, University of Bristol, Bristol, UK.ORCID 0000-0001-7307-2871
Sabine L FlitschManchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk.ORCID 0000-0003-3974-646X
Anthony P GreenManchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk.ORCID 0000-0003-0454-1798
Jolanda M van MunsterManchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk and Scotland's Rural College, Central Faculty, Edinburgh, UK.ORCID 0000-0003-0412-7001

Funding

Biotechnology and Biological Sciences Research Council BB/M027023/1
6 · The paper itself

Abstract

Promiscuous activity of a glycosyltransferase was exploited to polymerise glucose from UDP-glucose via the generation of β-1,4-glycosidic linkages. The biocatalyst was incorporated into biocatalytic cascades and chemo-enzymatic strategies to synthesise cello-oligosaccharides with tailored functionalities on a scale suitable for employment in mass spectrometry-based assays. The resulting glycan structures enabled reporting of the activity and selectivity of celluloltic enzymes.

Indexed as

GlycosyltransferasesMass Spectrometrybeta-GlucansBiocatalysisbeta-GlucansGlycosyltransferases

Identifiers

PMID34105582
PMCPMC8243248

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.