ArticleNature communications2021
The archaeal protein SepF is essential for cell division in Haloferax volcanii.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 42 citations in OpenAlex.
- Defining the order of assembly of theJournal of bacteriology · 2026Article
- Molecular Mechanism of the SepF N‑Terminal Conformational Switch in Ring Assembly and Membrane Anchoring.JACS Au · 2026Article
- A conserved oscillatory system that positions the divisome in Archaea.bioRxiv : the preprint server for biology · 2026Article
- Asgard archaea: have we found our microbial ancestors?The EMBO journal · 2026Review
- Defining the order of assembly of thebioRxiv : the preprint server for biology · 2026Article
- Article
- Distinct filament morphology and membrane tethering features of the dual FtsZ paralogs in Odinarchaeota.The EMBO journal · 2025Article
- CurT/CURT1 proteins are involved in cell and chloroplast division coordination of cyanobacteria and green algae.Nature communications · 2025Article
- Cell division protein CdpA organises and anchors the midcell ring in haloarchaea.Nature communications · 2025Article
- Article
- Identification of SepF in Streptococcus suis involving cell division.BMC microbiology · 2025Article
- Rhomboid proteases: key players at the cell surface within haloarchaea.Frontiers in microbiology · 2025Article
- Article
- Article
- The Archaeal Cell Cycle.Annual review of cell and developmental biology · 2024Review
- Extracellular vesicle formation inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Finding pieces in the archaeal cell division puzzle.Nature microbiology · 2024Article
- Proteins containing photosynthetic reaction centre domains modulate FtsZ-based archaeal cell division.Nature microbiology · 2024Article
- Widespread photosynthesis reaction centre barrel proteins are necessary for haloarchaeal cell division.Nature microbiology · 2024Article
- Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most bacteria, cell division depends on the tubulin homolog FtsZ and other proteins, such as SepF, that form a complex termed the divisome. Cell division also depends on FtsZ in many archaea, but other components of the divisome are unknown. Here, we demonstrate that a SepF homolog plays important roles in cell division in Haloferax volcanii, a halophilic archaeon that is known to have two FtsZ homologs with slightly different functions (FtsZ1 and FtsZ2). SepF co-localizes with both FtsZ1 and FtsZ2 at midcell. Attempts to generate a sepF deletion mutant were unsuccessful, suggesting an essential role. Indeed, SepF depletion leads to severe cell division defects and formation of large cells. Overexpression of FtsZ1-GFP or FtsZ2-GFP in SepF-depleted cells results in formation of filamentous cells with a high number of FtsZ1 rings, while the number of FtsZ2 rings is not affected. Pull-down assays support that SepF interacts with FtsZ2 but not with FtsZ1, although SepF appears delocalized in the absence of FtsZ1. Archaeal SepF homologs lack a glycine residue known to be important for polymerization and function in bacteria, and purified H. volcanii SepF forms dimers, suggesting that polymerization might not be important for the function of archaeal SepF.
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