ArticleCell discovery2021
2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism.
Article in Cell discovery, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- EngineeringACS synthetic biology · 2026Article
- Mixed sugars to a multipurpose chemical: perspectives of 2,3-butanediol fermentability in complex lignocellulose hydrolysates.Bioprocess and biosystems engineering · 2026Review
- Metabolic Profiling of Wheat Seedlings Under Oxygen Deficiency and Subsequent Reaeration Conditions.International journal of molecular sciences · 2025Article
- The impact of co-fed plastic diet on Tenebrio molitor gut bacterial community structure.Scientific reports · 2025Article
- Multidimensional Engineering of Escherichia coli for Efficient Adipic Acid Synthesis From Cyclohexane.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Bacterial membrane vesicles restore gut anaerobiosis.NPJ biofilms and microbiomes · 2025Article
- Monitoring the metabolite content of seasoned zucchinis during storage by NMR-based metabolomics.Heliyon · 2024Article
- Computational analysis into the potential of azo dyes as a feedstock for actinorhodin biosynthesis in Pseudomonas putida.PloS one · 2024Article
- Advances in the optimization of central carbon metabolism in metabolic engineering.Microbial cell factories · 2023Review
- Metabolic engineering for the production of acetoin and 2,3-butanediol at elevated temperature inFrontiers in bioengineering and biotechnology · 2023Article
- Highly efficient production of 2,3-butanediol from xylose and glucose by newly isolated thermotolerant Cronobacter sakazakii.BMC microbiology · 2022Article
- Non-Sterilized Fermentation of 2,3-Butanediol with Seawater by Metabolic Engineered Fast-GrowingFrontiers in bioengineering and biotechnology · 2022Article
- Metabolic engineering design to enhance (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis based on flux balance analysis.Microbial cell factories · 2021Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic process with unidentified functions. We demonstrate here that acetate increases and then decreases during the growth of a bacterium Enterobacter cloacae subsp. dissolvens SDM. Both bifunctional acetaldehyde/ethanol dehydrogenase AdhE-catalyzed ethanol production and acetate-induced 2,3-BD biosynthesis are indispensable for the elimination of acetate generated during overflow metabolism. 2,3-BD biosynthesis from glucose supplies NADH required for acetate elimination via AdhE-catalyzed ethanol production. The coupling strategy involving 2,3-BD biosynthesis and ethanol production is widely distributed in bacteria and is important for toxic acetate elimination. Finally, we realized the co-production of ethanol and acetoin from chitin, the second most abundant natural biopolymer whose catabolism involves inevitable acetate production through the coupling acetate elimination strategy. The synthesis of a non-toxic chemical such as 2,3-BD may be viewed as a unique overflow metabolism with desirable metabolic functions.
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Registered trials
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