Evidence map›Paper›PMID 34103026›Full record

ArticleBMC medicine2021

Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke.

Takaki Hiwasa, Hao Wang, Ken-Ichiro Goto, Seiichiro Mine, Toshio Machida, Eiichi Kobayashi, Yoichi Yoshida, Akihiko Adachi, Tomoo Matsutani, Mizuki Sata and 60 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 28 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

70 authors at 10 institutions in 2 countries.

Takaki HiwasaDepartment of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan. hiwasa_takaki@faculty.chiba-u.jp.ORCID 0000-0002-0475-3881
Hao WangDepartment of Biochemistry and Genetics, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Ken-Ichiro GotoDepartment of Biochemistry and Genetics, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Seiichiro MineDepartment of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Toshio MachidaDepartment of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Eiichi KobayashiDepartment of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Yoichi YoshidaDepartment of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Akihiko AdachiDepartment of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Tomoo MatsutaniDepartment of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Mizuki SataDepartment of Public Health Medicine, Faculty of Medicine, and Health Services Research and Development Center, University of Tsukuba, Tsukuba, 305-8575, Japan.
Kazumasa YamagishiDepartment of Public Health Medicine, Faculty of Medicine, and Health Services Research and Development Center, University of Tsukuba, Tsukuba, 305-8575, Japan.
Hiroyasu IsoPublic Health, Department of Social Medicine, Osaka University Graduate School of Medicine, Suita, 565-0871, Japan.
Norie SawadaEpidemiology and Prevention Group, Center for Public Health Sciences, National Cancer Center, Tokyo, 104-0045, Japan.
Shoichiro TsuganeEpidemiology and Prevention Group, Center for Public Health Sciences, National Cancer Center, Tokyo, 104-0045, Japan.
Mitoshi KunimatsuDepartment of Home Economics, Nagoya Women's University, Nagoya, 467-8610, Japan.
Ikuo KamitsukasaDepartment of Neurology, Chiba Rosai Hospital, Chiba, 290-0003, Japan.
Masahiro MoriDepartment of Neurology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Kazuo SugimotoDepartment of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Akiyuki UzawaComprehensive Stroke Center, Chiba University Hospital, Chiba, 260-8677, Japan.
Mayumi MutoDepartment of Neurology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Satoshi KuwabaraComprehensive Stroke Center, Chiba University Hospital, Chiba, 260-8677, Japan.
Yoshio KobayashiDepartment of Cardiovascular Medicine, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Mikiko OhnoDepartment of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan.
Eiichiro NishiDepartment of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan.
Akiko HattoriDepartment of Endocrinology, Hematology and Gerontology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Masashi YamamotoDepartment of Endocrinology, Hematology and Gerontology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Yoshiro MaezawaDepartment of Endocrinology, Hematology and Gerontology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Kazuki KobayashiDepartment of Endocrinology, Hematology and Gerontology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Ryoichi IshibashiDepartment of Endocrinology, Hematology and Gerontology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Minoru TakemotoDepartment of Endocrinology, Hematology and Gerontology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Koutaro YokoteDepartment of Endocrinology, Hematology and Gerontology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Hirotaka TakizawaPort Square Kashiwado Clinic, Kashiwado Memorial Foundation, Chiba, 260-0025, Japan.
Takashi KishimotoDepartment of Molecular Pathology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Kazuyuki MatsushitaDepartment of Laboratory Medicine & Division of Clinical Genetics, Chiba University Hospital, Chiba, 260-8677, Japan.
Sohei KobayashiDepartment of Laboratory Medicine & Division of Clinical Genetics, Chiba University Hospital, Chiba, 260-8677, Japan.
Fumio NomuraDivision of Clinical Genetics, Chiba Foundation for Health Promotion and Disease Prevention, Chiba, 261-0002, Japan.
Takahiro ArasawaDepartment of Biochemistry and Genetics, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Akiko KagayaDepartment of Frontier Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Tetsuro MaruyamaDepartment of Frontier Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Hisahiro MatsubaraDepartment of Frontier Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Minako TomiitaDepartment of Allergy and Rheumatology, Chiba Children's Hospital, Chiba, 266-0007, Japan.
Shinsaku HamanakaDepartment of Gastroenterology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Yushi ImaiDepartment of Gastroenterology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Tomoo NakagawaDepartment of Gastroenterology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Naoya KatoDepartment of Gastroenterology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Jiro TeradaDepartment of Respirology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Takuma MatsumuraDepartment of Respirology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Yusuke KatsumataDepartment of Respirology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Akira NaitoDepartment of Respirology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Nobuhiro TanabeDepartment of Respirology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Seiichiro SakaoDepartment of Respirology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Koichiro TatsumiDepartment of Respirology, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Masaaki ItoDepartment of Gastroenterological Surgery and Clinical Oncology, Toho University Graduate School of Medicine, Tokyo, 143-8541, Japan.
Fumiaki ShiratoriDepartment of Gastroenterological Surgery and Clinical Oncology, Toho University Graduate School of Medicine, Tokyo, 143-8541, Japan.
Makoto SumazakiDepartment of Gastroenterological Surgery and Clinical Oncology, Toho University Graduate School of Medicine, Tokyo, 143-8541, Japan.
Satoshi YajimaDepartment of Gastroenterological Surgery and Clinical Oncology, Toho University Graduate School of Medicine, Tokyo, 143-8541, Japan.
Hideaki ShimadaDepartment of Gastroenterological Surgery and Clinical Oncology, Toho University Graduate School of Medicine, Tokyo, 143-8541, Japan.
Mikako ShirouzuDivision of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, Yokohama, Kanagawa, 230-0045, Japan.
Shigeyuki YokoyamaRIKEN Structural Biology Laboratory, Yokohama, Kanagawa, 230-0045, Japan.
Takashi KudoCelish FD Inc., Chiba, Japan.
Hirofumi DoiCelish FD Inc., Chiba, Japan.
Katsuro IwaseDepartment of Biochemistry and Genetics, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Hiromi AshinoDepartment of Biochemistry and Genetics, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Shu-Yang LiDepartment of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Masaaki KubotaDepartment of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Go TomiyoshiDepartment of Biochemistry and Genetics, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Natsuko ShinmenDepartment of Biochemistry and Genetics, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Rika NakamuraDepartment of Biochemistry and Genetics, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Hideyuki KurodaMedical Project Division, Research Development Center, Fujikura Kasei Co., Saitama, 340-0203, Japan.
Yasuo IwadateDepartment of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba, 260-8670, Japan.
Chiba University · JPToho University · JPChiba University Hospital · JPShiga University of Medical Science · JPUniversity of Tsukuba · JPChiba Hospital · JPChibaken Saiseikai Narashino Hospital · JPFujikura (Japan) · JPNagoya Women's University · JPThe University of Osaka · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAcute ischemic stroke (AIS) is a serious cause of mortality and disability. AIS is a serious cause of mortality and disability. Early diagnosis of atherosclerosis, which is the major cause of AIS, allows therapeutic intervention before the onset, leading to prevention of AIS.

methodsSerological identification by cDNA expression cDNA libraries and the protein array method were used for the screening of antigens recognized by serum IgG antibodies in patients with atherosclerosis. Recombinant proteins or synthetic peptides derived from candidate antigens were used as antigens to compare serum IgG levels between healthy donors (HDs) and patients with atherosclerosis-related disease using the amplified luminescent proximity homogeneous assay-linked immunosorbent assay.

resultsThe first screening using the protein array method identified death-inducer obliterator 1 (DIDO1), forkhead box J2 (FOXJ2), and cleavage and polyadenylation specificity factor (CPSF2) as the target antigens of serum IgG antibodies in patients with AIS. Then, we prepared various antigens including glutathione S-transferase-fused DIDO1 protein as well as peptides of the amino acids 297-311 of DIDO1, 426-440 of FOXJ2, and 607-621 of CPSF2 to examine serum antibody levels. Compared with HDs, a significant increase in antibody levels of the DIDO1 protein and peptide in patients with AIS, transient ischemic attack (TIA), and chronic kidney disease (CKD) but not in those with acute myocardial infarction and diabetes mellitus (DM). Serum anti-FOXJ2 antibody levels were elevated in most patients with atherosclerosis-related diseases, whereas serum anti-CPSF2 antibody levels were associated with AIS, TIA, and DM. Receiver operating characteristic curves showed that serum DIDO1 antibody levels were highly associated with CKD, and correlation analysis revealed that serum anti-FOXJ2 antibody levels were associated with hypertension. A prospective case-control study on ischemic stroke verified that the serum antibody levels of the DIDO1 protein and DIDO1, FOXJ2, and CPSF2 peptides showed significantly higher odds ratios with a risk of AIS in patients with the highest quartile than in those with the lowest quartile, indicating that these antibody markers are useful as risk factors for AIS.

conclusionsSerum antibody levels of DIDO1, FOXJ2, and CPSF2 are useful in predicting the onset of atherosclerosis-related AIS caused by kidney failure, hypertension, and DM, respectively.

Indexed as

AntibodiesBrain IschemiaIschemic StrokeStrokeCase-Control StudiesCleavage And Polyadenylation Specificity FactorDNA-Binding ProteinsForkhead Transcription FactorsHumansAntibodiesCleavage And Polyadenylation Specificity FactorCPSF2 protein, humanDIDO1 protein, humanDNA-Binding ProteinsForkhead Transcription FactorsFOXJ2 protein, humanAcute ischemic strokeAcute myocardial infarctionAntibody biomarkerAtherosclerosisChronic kidney diseaseDiabetes mellitus

Identifiers

PMID34103026
PMCPMC8188684
OpenAlexW3170034496

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.