ArticleJournal of the American Society for Mass Spectrometry2021
Microdroplet Mass Spectrometry Enables Extremely Accelerated Pepsin Digestion of Proteins.
Article in Journal of the American Society for Mass Spectrometry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- A Unified Mass Spectrometry Workflow Integrating Bottom-Up and Native Top-Down Proteomics via Nanodroplet Enzymatic Digestion.Analytical chemistry · 2026Article
- Rapid Antibody Structural Characterization and Quantification via Microdroplet Trypsin Digestion.Journal of the American Society for Mass Spectrometry · 2026Article
- Atmospheric Sampling Mass Spectrometers Activate and Ionize Neutral Water Microdroplets to MeV Energies and up to 200,000+ Charges: Implications for Water Stability and Unusual Chemistry in Microdroplets.ACS central science · 2025Article
- Droplet-on-demand mass spectrometry reveals curvature-dependent interfacial reactivity in aqueous microdroplets.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mechanisms of ionization and of chemical reactions in charged microdroplets.Chemical science · 2025Review
- Ultrafast Microdroplet Digestion of Antibodies with Fc-Silencing Mutations.Analytical chemistry · 2025Article
- Rapid Characterization of Antibodies via Automated Flow Injection Coupled with Online Microdroplet Reactions and Native-pH Mass Spectrometry.Analytical chemistry · 2023Article
- Investigation of Tryptic Protein Digestion in Microdroplets and in Bulk Solution.Journal of the American Society for Mass Spectrometry · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In microdroplets, rates of chemical or biomolecular reactions can exceed those in the bulk phase by more than a million times. As electrospray ionization-based mass spectrometry (MS) involves the formation of charged microdroplets, reaction acceleration and online MS monitoring of reaction products can readily be performed at the same time. We investigated accelerated enzymatic reactions in microdroplets and focused on the proteolytic enzyme pepsin. Electrosonic spray ionization (ESSI) was utilized for developing the ultrarapid pepsin in-spray digestion of two different proteins, cytochrome
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Registered trials
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