ArticleJournal of industrial microbiology & biotechnology2021
Streptomyces venezuelae NRRL B-65442: genome sequence of a model strain used to study morphological differentiation in filamentous actinobacteria.
Article in Journal of industrial microbiology & biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Review
- Inactivation of the sco2730/2731 copper chaperone-transporter system in Streptomyces coelicolor and its orthologs in Streptomyces venezuelae, together with chromosomal end deletion, greatly enhances secondary metabolism.Microbial cell factories · 2026Article
- A Simple and Versatile Cell-Free Expression Method for Producing Secondary Metabolites.ACS synthetic biology · 2026Article
- Streptomyces uses both polar and dispersed cell wall synthesis during exploratory growth.Nature microbiology · 2025Article
- AdpA, a Global Regulator of Hundreds of Genes, Including Those for Secondary Metabolism, inAntibiotics (Basel, Switzerland) · 2025Article
- Article
- Impact of the alkaline volatile trimethylamine on the physiology ofMicrobiology spectrum · 2025Article
- Application of multiple genomic-editing technologies inSynthetic and systems biotechnology · 2025Article
- Deletion ofMicrobiology (Reading, England) · 2025Article
- Microbe Profile:Microbiology (Reading, England) · 2025Review
- A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division.BMC microbiology · 2024Article
- The Best of Both Worlds-Streptomyces coelicolor and Streptomyces venezuelae as Model Species for Studying Antibiotic Production and Bacterial Multicellular Development.Journal of bacteriology · 2023Review
- Evidence of a role for CutRS and actinorhodin in the secretion stress response inMicrobiology (Reading, England) · 2023Article
- Global Effects of the Developmental Regulator BldB in Streptomyces venezuelae.Journal of bacteriology · 2023Article
- Cost-effective hybrid long-short read assembly delineates alternative GC-richSynthetic and systems biotechnology · 2023Article
- Genome-Wide Identification of the LexA-Mediated DNA Damage Response in Streptomyces venezuelae.Journal of bacteriology · 2022Article
- Genome mining for drug discovery: progress at the front end.Journal of industrial microbiology & biotechnology · 2021Review
- AdpA Positively Regulates Morphological Differentiation and Chloramphenicol Biosynthesis in Streptomyces venezuelae.Microbiology spectrum · 2021Article
- Bilateral symmetry of linear streptomycete chromosomes.Microbial genomics · 2021Article
- Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic development.Nature communications · 2021Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
For over a decade, Streptomyces venezuelae has been used to study the molecular mechanisms that control morphological development in streptomycetes and is now a well-established model strain. Its rapid growth and ability to sporulate in a near-synchronised manner in liquid culture, unusual among streptomycetes, greatly facilitates the application of modern molecular techniques such as ChIP-seq and RNA-seq, as well as time-lapse fluorescence imaging of the complete Streptomyces life cycle. Here we describe a high-quality genome sequence of our isolate of the strain (Northern Regional Research Laboratory [NRRL] B-65442) consisting of an 8.2 Mb chromosome and a 158 kb plasmid, pSVJI1, which had not been reported previously. Surprisingly, while NRRL B-65442 yields green spores on MYM agar, the American Type Culture Collection (ATCC) type strain 10712 (from which NRRL B-65442 was derived) produces grey spores. While comparison of the genome sequences of the two isolates revealed almost total identity, it did reveal a single nucleotide substitution in a gene, vnz_33525, involved in spore pigment biosynthesis. Replacement of the vnz_33525 allele of ATCC 10712 with that of NRRL B-65442 resulted in green spores, explaining the discrepancy in spore pigmentation. We also applied CRISPR-Cas9 to delete the essential parB of pSVJI1 to cure the plasmid from the strain without obvious phenotypic consequences.
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