Evidence map›Paper›PMID 34100897›Full record

ArticleBiology open2021

MICAL-L1 is required for cargo protein delivery to the cell surface.

R Sikora, P Bun, L Danglot, M Alqabandi, P Bassereau, F Niedergang, T Galli, A Zahraoui

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology open, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

R SikoraUniversité de Paris, Inserm U1016-CNRS UMR 8104, Institut Cochin, Paris, France.
P BunUniversité de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Membrane Traffic in Healthy & Diseased Brain, Paris, France.
L DanglotUniversité de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Membrane Traffic in Healthy & Diseased Brain, Paris, France.ORCID 0000-0001-6190-6605
M AlqabandiLaboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS, UMR168, 75005, Paris, France.
P BassereauLaboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS, UMR168, 75005, Paris, France.ORCID 0000-0002-8544-6778
F NiedergangUniversité de Paris, Inserm U1016-CNRS UMR 8104, Institut Cochin, Paris, France.
T GalliUniversité de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Membrane Traffic in Healthy & Diseased Brain, Paris, France.ORCID 0000-0001-8514-7455
A ZahraouiUniversité de Paris, Inserm U1016-CNRS UMR 8104, Institut Cochin, Paris, France.ORCID 0000-0001-7424-8322
Centre National de la Recherche Scientifique · FRInserm · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Secreted proteins are transported along intracellular route from the endoplasmic reticulum through the Golgi before reaching the plasma membrane. Small GTPase Rab and their effectors play a key role in membrane trafficking. Using confocal microscopy, we showed that MICAL-L1 was associated with tubulo-vesicular structures and exhibited a significant colocalization with markers of the Golgi apparatus and recycling endosomes. Super resolution STORM microscopy suggested at the molecular level, a very close association of MICAL-L1 and microdomains in the Golgi cisternae. Using a synchronized secretion assay, we report that the shRNA-mediated depletion of MICAL-L1 impaired the delivery of a subset of cargo proteins to the cell surface. The process of membrane tubulation was monitored in vitro, and we observe that recombinant MICAL-L1-RBD domain may contribute to promote PACSINs-mediated membrane tubulation. Interestingly, two hydrophobic residues at the C-terminus of MICAL-L1 appeared to be important for phosphatidic acid binding, and for association with membrane tubules. Our results reveal a new role for MICAL-L1 in cargo delivery to the plasma membrane.

Indexed as

Amino AcidsBinding SitesCalponinsCell LineCell MembraneFluorescent Antibody TechniqueHeLa CellsHumansImmunohistochemistryMicrofilament ProteinsMixed Function OxygenasesProtein BindingProtein Interaction Domains and MotifsProtein TransportAmino AcidsCalponinsMICAL1 protein, humanMicrofilament ProteinsMixed Function OxygenasesMembrane trafficMembrane tubulesMICAL-L1Rab effectors

Identifiers

PMID34100897
PMCPMC8214422
OpenAlexW3168075711

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.