Evidence map›Paper›PMID 34100012›Full record

ArticlebioRxiv : the preprint server for biology2021

SARS-CoV-2 infection studies in lung organoids identify TSPAN8 as novel mediator.

Lisiena Hysenaj, Samantha Little, Kayla Kulhanek, Oghenekevwe M Gbenedio, Lauren Rodriguez, Alan Shen, Jean-Christophe Lone, Leonard C Lupin-Jimenez, Luke R Bonser, Nina K Serwas and 18 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Biochemical and mechanical regulation of actin dynamics.Nature reviews. Molecular cell biology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors at 3 institutions in 2 countries.

Lisiena HysenajDepartment of Anatomy, University of California, San Francisco, San Francisco, California 94143, USA.
Samantha LittleDepartment of Anatomy, University of California, San Francisco, San Francisco, California 94143, USA.
Kayla KulhanekDepartment of Anatomy, University of California, San Francisco, San Francisco, California 94143, USA.
Oghenekevwe M GbenedioDepartment of Anatomy, University of California, San Francisco, San Francisco, California 94143, USA.
Lauren RodriguezDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, California 94143, USA.
Alan ShenUCSF CoLabs, University of California San Francisco, San Francisco, California 94143, USA.
Jean-Christophe LoneSchool of Life Science, University of Essex, Wivenhoe Park,Colchester C04 3SQ, United Kingdom.
Leonard C Lupin-JimenezUCSF CoLabs, University of California San Francisco, San Francisco, California 94143, USA.
Luke R BonserLung Biology Center, Department of Medicine, University of California, San Francisco, San Francisco, California 94143, USA.
Nina K SerwasDepartment of Pathology, University of California, San Francisco, San Francisco, California 94143, USA.
Kriti BahlDepartment of Anatomy, University of California, San Francisco, San Francisco, California 94143, USA.
Eran MickDivision of Infectious Diseases, University of California, San Francisco, San Francisco, California 94143, USA and Department of Surgery, Division of Cardiothoracic Surgery, University of California, San Francisco, San Francisco, California 94143, USA.
Jack Z LiCardiovascular Research Institute, Departments of Medicine and Anesthesia, University of California, San Francisco, San Francisco, California 94143, USA.
Vivianne W DingCardiovascular Research Institute, Departments of Medicine and Anesthesia, University of California, San Francisco, San Francisco, California 94143, USA.
Shotaro MatsumotoGladstone Institute of Virology, Department of Medicine, University of California San Francisco, California 94143, USA.
Mazharul MaishanGladstone Institute of Virology, Department of Medicine, University of California San Francisco, California 94143, USA.
Camille SimoneauDepartment of Medicine, Division of Rheumatology, University of California, San Francisco, San Francisco, California 94143, USA.
Gabriela FragiadakisUCSF CoLabs, University of California San Francisco, San Francisco, California 94143, USA.
David M JablonsCardiovascular Research Institute, Departments of Medicine and Anesthesia, University of California, San Francisco, San Francisco, California 94143, USA.
Charles R LangelierDivision of Infectious Diseases, University of California, San Francisco, San Francisco, California 94143, USA and Department of Surgery, Division of Cardiothoracic Surgery, University of California, San Francisco, San Francisco, California 94143, USA.
Michael MatthayGladstone Institute of Virology, Department of Medicine, University of California San Francisco, California 94143, USA.
Melanie OttDepartment of Medicine, Division of Rheumatology, University of California, San Francisco, San Francisco, California 94143, USA.
Matthew KrummelDepartment of Pathology, University of California, San Francisco, San Francisco, California 94143, USA.
Alexis J CombesUCSF CoLabs, University of California San Francisco, San Francisco, California 94143, USA.
Anita SilDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, California 94143, USA.
David J ErleUCSF CoLabs, University of California San Francisco, San Francisco, California 94143, USA.
Johannes R KratzCardiovascular Research Institute, Departments of Medicine and Anesthesia, University of California, San Francisco, San Francisco, California 94143, USA.
Jeroen P RooseDepartment of Anatomy, University of California, San Francisco, San Francisco, California 94143, USA.
University of California, San Francisco · USGladstone Institutes · USUniversity of Essex · GB

Funding

Understanding Asthma EndotypesU19AI077439 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI PRESCOTT G WOODRUFF · 2008 to 2026
$41.1M
Understand the metabolic fitness of naïve T cellsP01AI091580 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JEROEN ROOSE · 2011 to 2026
$31.1M
Airway epithelial cell gene regulation: new mechanisms and therapeutic strategiesR35HL145235 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ERLE, DAVID J · 2019 to 2025
$6.8M
Microbial Pathogenesis and Host DefenseT32AI060537 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Joanne N. Engel · 2004 to 2026
$6.5M
NHLBI NIH HHS R35 HL145235NIAID NIH HHS P01 AI091580NIAID NIH HHS T32 AI060537NIAID NIH HHS U19 AI077439
6 · The paper itself

Abstract

SARS coronavirus-2 (SARS-CoV-2) is causing a global pandemic with large variation in COVID-19 disease spectrum. SARS-CoV-2 infection requires host receptor ACE2 on lung epithelium, but epithelial underpinnings of variation are largely unknown. We capitalized on comprehensive organoid assays to report remarkable variation in SARS-CoV-2 infection rates of lung organoids from different subjects. Tropism is highest for TUBA- and MUC5AC-positive organoid cells, but levels of TUBA-, MUC5A-, or ACE2- positive cells do not predict infection rate. We identify surface molecule Tetraspanin 8 (TSPAN8) as novel mediator of SARS-CoV-2 infection, which is not downregulated by this specific virus. TSPAN8 levels, prior to infection, strongly correlate with infection rate and TSPAN8-blocking antibodies diminish SARS-CoV-2 infection. We propose TSPAN8 as novel functional biomarker and potential therapeutic target for COVID-19.

Identifiers

PMID34100012
PMCPMC8183007
OpenAlexW3165694026

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.