ReviewNature methods2021
The emerging landscape of single-molecule protein sequencing technologies.
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In one paragraph
Review in Nature methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 168 papers.
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168citing papers in PubMed
–field-weighted citation impact
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Who cites it
168 citing papers in PubMed.
- Article
- Engineering a Biological Nanopore for Monitoring Protein Dynamics and Conformational Changes at the Single-Molecule Level.ACS nano · 2026Article
- High-resolution nanopore peptide sensing, profiling and sequence assembly.Nature nanotechnology · 2026Article
- High-Rate Fingerprinting of Protein Isoforms by Quasi-regulated Enzyme-free Transport Through CytK Nanopores.Research square · 2026Article
- Optimization of the Quantum-Si Platinum Single-Molecule Protein Sequencing Platform Toward Improved Complex-Matrix Protein Identification.ACS omega · 2026Article
- Immunopeptidomics-guided identification of functional neoantigens in non-small cell lung cancer.NPJ precision oncology · 2026Article
- Sequencing peptides by reversing translation.Nature biotechnology · 2026Article
- Digital Immunoassays for Sensitive Quantification of Blood Biomarkers Using Solid-State Nanopores.ACS nano · 2026Article
- Proteoform medicine: characterizing and targeting protein forms in human disease.Nature reviews. Genetics · 2026Review
- Label-Free Optical Differentiation of Single Diffusing Amino Acids at Picomolar Concentrations.Research square · 2026Article
- Single-molecule peptide sequencing through reverse translation of peptides into DNA.Nature biotechnology · 2026Article
- Proteoforms as the true units of physiological function.European journal of applied physiology · 2026Review
- Uncovering Hidden Protein Conformations with High Bandwidth Nanopore Measurements.Nano letters · 2026Article
- Nanopore-based massively parallel sensing for peptide profiling and protein identification.Nature communications · 2026Article
- Principles ofbioRxiv : the preprint server for biology · 2026Article
- Single-molecule electrical protein fingerprinting in solid-state nanopores.Clinical and translational medicine · 2026Article
- Optoelectronic Tweezers for Single-Cell Research: Principles, Applications, and Prospects.Cyborg and bionic systems (Washington, D.C.) · 2026Review
- Threading of Single Protein Molecules Through a Solid-State Nanopore.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Automated Intelligent Microscopy for Phenotype Identification, Spatial Localization, and Retargeting of Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Protein abundance inference via expectation-maximization in fluorosequencing.Bioinformatics advances · 2026Article
108 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
43 authors.
Javier Antonio Alfaro *International Centre for Cancer Vaccine Science, University of Gdańsk, Gdańsk, Poland. javier.alfaro@proteogenomics.ca.ORCID http://orcid.org/0000-0002-5553-6991
Peggy Bohländer *Faculty of Applied Sciences, Delft University of Technology, Delft, the Netherlands.
Mingjie Dai *Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0002-8665-4966
Mike Filius *Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands.ORCID http://orcid.org/0000-0002-5434-8358
Cecil J Howard *Department of Chemistry, University of Texas at Austin, Austin, TX, USA.
Xander F van Kooten *Department of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
Shilo Ohayon *Department of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
Adam Pomorski *Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands.ORCID http://orcid.org/0000-0002-0669-3421
Sonja Schmid *NanoDynamicsLab, Laboratory of Biophysics, Wageningen University, Wageningen, the Netherlands.ORCID http://orcid.org/0000-0002-3710-5602
Aleksei AksimentievDepartment of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, USA.ORCID http://orcid.org/0000-0002-6042-8442
Eric V AnslynDepartment of Chemistry, University of Texas at Austin, Austin, TX, USA.ORCID http://orcid.org/0000-0002-5137-8797
Georges BedranInternational Centre for Cancer Vaccine Science, University of Gdańsk, Gdańsk, Poland.
Chan CaoInstitute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.ORCID http://orcid.org/0000-0003-2592-0690
Mauro ChinappiDipartimento di Ingegneria Industriale, Università di Roma Tor Vergata, Rome, Italy.
Etienne CoyaudUniv. Lille, Inserm, CHU Lille, U1192-Protéomique Réponse Inflammatoire Spectrométrie de Masse-PRISM, Lille, France.
Cees DekkerDepartment of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands.
Gunnar DittmarDepartment of Infection and Immunity, Luxembourg Institute of Health, Strassen, Luxembourg.
Nicholas DrachmanDepartment of Physics, Brown University, Providence, RI, USA.
Rienk EelkemaFaculty of Applied Sciences, Delft University of Technology, Delft, the Netherlands.
David GoodlettInternational Centre for Cancer Vaccine Science, University of Gdańsk, Gdańsk, Poland.
Sébastien HentzUniversité Grenoble Alpes, CEA, LETI, Grenoble, France.ORCID http://orcid.org/0000-0003-0479-9027
Umesh KalathiyaInternational Centre for Cancer Vaccine Science, University of Gdańsk, Gdańsk, Poland.ORCID http://orcid.org/0000-0001-7757-6962
Neil L KelleherDepartments of Chemistry and Molecular Biosciences, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.ORCID http://orcid.org/0000-0002-8815-3372
Ryan T KellyDepartment of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.
Zvi KelmanInstitute for Bioscience and Biotechnology Research, National Institute of Standards and Technology, University of Maryland, Rockville, MD, USA.
Sung Hyun KimDepartment of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands.
Bernhard KusterChair of Proteomics and Bioanalytics, Technische Universität München, Freising, Germany.ORCID http://orcid.org/0000-0002-9094-1677
David Rodriguez-LarreaDepartment of Biochemistry and Molecular Biology, Biofisika Institute (CSIC, UPV/EHU), Leioa, Spain.ORCID http://orcid.org/0000-0002-5580-7413
Stuart LindsayBiodesign Institute, School of Molecular Sciences, Department of Physics, Arizona State University, Tempe, AZ, USA.
Giovanni MagliaGroningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands.ORCID http://orcid.org/0000-0003-2784-0811
Edward M MarcotteDepartment of Molecular Biosciences, Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, USA.
John P MarinoInstitute for Bioscience and Biotechnology Research, National Institute of Standards and Technology, University of Maryland, Rockville, MD, USA.ORCID http://orcid.org/0000-0002-6860-5853
Christophe MasselonUniversité Grenoble Alpes, CEA, Inserm, BGE U1038, Grenoble, France.ORCID http://orcid.org/0000-0003-0117-2480
Michael MayerAdolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland.
Patroklos SamarasChair of Proteomics and Bioanalytics, Technische Universität München, Freising, Germany.ORCID http://orcid.org/0000-0001-6042-1585
Kumar SarthakDepartment of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Lusia SepiashviliUniversity of Toronto, Hospital for Sick Children, Toronto, Ontario, Canada.
Derek SteinDepartment of Physics, Brown University, Providence, RI, USA.ORCID http://orcid.org/0000-0001-5917-4986
Meni WanunuDepartment of Physics, Northeastern University, Boston, MA, USA.
Mathias WilhelmChair of Proteomics and Bioanalytics, Technische Universität München, Freising, Germany.ORCID http://orcid.org/0000-0002-9224-3258
Peng YinWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.ORCID http://orcid.org/0000-0002-2769-6357
Amit Meller *Department of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel. ameller@technion.ac.il.ORCID http://orcid.org/0000-0001-7082-0985
Chirlmin Joo *Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands. c.joo@tudelft.nl.ORCID http://orcid.org/0000-0003-2803-0335
Funding
TR&D 7: Cell Specific ProteomicsP41GM108569 · NIGMS · NORTHWESTERN UNIVERSITY · PI KELLEHER, NEIL L · 2015 to 2024
$13.6MHigh-throughput single-molecule protein identification via super-resolution imagingDP1GM133052 · NIGMS · HARVARD UNIVERSITY · PI YIN, PENG · 2018 to 2022
$6.6MProteomics and model organism humanization to decode human geneticsR35GM122480 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI EDWARD M MARCOTTE · 2017 to 2026
$5.4MSuper-resolution imaging via programmable autonomous blinkingR01EB018659 · NIBIB · HARVARD MEDICAL SCHOOL · PI YIN, PENG · 2013 to 2017
$3.3MSingle-cell direct RNA sequencing using electrical zero-mode waveguides and engineered reverse transcriptasesR01HG011087 · NHGRI · NORTHEASTERN UNIVERSITY · PI WANUNU, MENI · 2020 to 2023
$3.0MEngineering Bioimaging Probes Based on Triggered Molecular GeometryDP2OD007292 · OD · HARVARD MEDICAL SCHOOL · PI YIN, PENG · 2010 to 2010
$2.5MDirect picogram DNA and RNA sequencing using nanopore Zero-mode waveguidesR01HG009186 · NHGRI · NORTHEASTERN UNIVERSITY · PI WANUNU, MENI · 2016 to 2019
$2.3MFully automated and ultra-high-throughput platform for in-depth single-cell proteomicsR01GM138931 · NIGMS · BRIGHAM YOUNG UNIVERSITY · PI KELLY, RYAN T · 2020 to 2023
$1.6MRenewable and Specific Affinity Reagents for Mapping Proteoforms in Human TissuesUH3CA246635 · NCI · NORTHWESTERN UNIVERSITY · PI KELLEHER, NEIL L · 2019 to 2021
$1.4MMapping the blood cell protein complexosomeR01DK110520 · NIDDK · UNIVERSITY OF TEXAS AT AUSTIN · PI EMILI, ANDREW, MARCOTTE, EDWARD M · 2016 to 2018
$727kNCI NIH HHS UH3 CA246635NHGRI NIH HHS R01 HG009186NHGRI NIH HHS R01 HG011087NIBIB NIH HHS R01 EB018659NIDDK NIH HHS R01 DK110520NIGMS NIH HHS DP1 GM133052NIGMS NIH HHS P41 GM108569NIGMS NIH HHS R01 GM138931NIGMS NIH HHS R35 GM122480NIH HHS DP2 OD007292
Abstract
Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.
Indexed as
Mass SpectrometryNanotechnologyProteinsProteomicsSequence Analysis, ProteinSequence Analysis, RNASingle-Cell AnalysisSingle Molecule ImagingProteins
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