ArticleNature communications2021
Structural-profiling of low molecular weight RNAs by nanopore trapping/translocation using Mycobacterium smegmatis porin A.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- A universal deep learning framework for empowering nanopore identification by reinforcing temporal signals.Nature communications · 2026Article
- tRNA modifications in cancer: from molecular mechanisms to clinical translation.Biomarker research · 2026Review
- Review
- Nanopore sequencing of intact aminoacylated tRNAs.Nature communications · 2025Article
- Nanopore discrimination of rare earth elements.Nature nanotechnology · 2025Article
- Impact of MSMEG5257 Deletion onMicroorganisms · 2024Article
- Nanopore analysis of cis-diols in fruits.Nature communications · 2024Article
- The C-terminus is essential for the stability of the mycobacterial channel protein MspA.Protein science : a publication of the Protein Society · 2024Article
- T-S2Inet: Transformer-based sequence-to-image network for accurate nanopore sequence recognition.Bioinformatics (Oxford, England) · 2024Article
- Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing.Nature biotechnology · 2024Article
- Quantitation of CirculatingACS nano · 2023Article
- Real-time label-free detection of dynamic aptamer-small molecule interactions using a nanopore nucleic acid conformational sensor.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cell-Free Expression ofACS nano · 2023Article
- Machine learning for small interfering RNAs: a concise review of recent developments.Frontiers in genetics · 2023Review
- Active learning for efficient analysis of high-throughput nanopore data.Bioinformatics (Oxford, England) · 2023Article
- A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples.Nature communications · 2022Article
- Nanopore Detection Assisted DNA Information Processing.Nanomaterials (Basel, Switzerland) · 2022Review
- Translocation Behaviors of Synthetic Polyelectrolytes through Alpha-Hemolysin (α-HL) andJournal of the Electrochemical Society · 2022Article
- Promising Assays for Examining a Putative Role of Ribosomal Heterogeneity in COVID-19 Susceptibility and Severity.Life (Basel, Switzerland) · 2022Review
- Article
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Folding of RNA can produce elaborate tertiary structures, corresponding to their diverse roles in the regulation of biological activities. Direct observation of RNA structures at high resolution in their native form however remains a challenge. The large vestibule and the narrow constriction of a Mycobacterium smegmatis porin A (MspA) suggests a sensing mode called nanopore trapping/translocation, which clearly distinguishes between microRNA, small interfering RNA (siRNA), transfer RNA (tRNA) and 5 S ribosomal RNA (rRNA). To further profit from the acquired event characteristics, a custom machine learning algorithm is developed. Events from measurements with a mixture of RNA analytes can be automatically classified, reporting a general accuracy of ~93.4%. tRNAs, which possess a unique tertiary structure, report a highly distinguishable sensing feature, different from all other RNA types tested in this study. With this strategy, tRNAs from different sources are measured and a high structural conservation across different species is observed in single molecule.
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