ArticleNature communications2021
Multiplexed live-cell profiling with Raman probes.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
- Longitudinal live-cell chemical imaging using coherent Raman scattering microscopy.The Analyst · 2026Review
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Machine-Learning-Enabled Raman Spectroscopy Refines Indocyanine Green Fluorescence Boundaries for Precise Glioblastoma Margin Delineation.Research (Washington, D.C.) · 2026Article
- Raman imaging of molecular groups in the wavenumber silent region.Analytical and bioanalytical chemistry · 2026Review
- Stimulated Raman Scattering Imaging Enabled Biomarker Discovery for Precision Medicine.Chemical & biomedical imaging · 2025Review
- Bond-Selective Imaging at the Frontier of Biomedicine.Chemical & biomedical imaging · 2025Article
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Label-Free Longitudinal Imaging of Single Cell Drug Response with a 3D-Printed Cell Culture Platform.Analytical chemistry · 2025Article
- Confocal Raman microspectroscopy for spatially resolved tissue characterisation of disease-linked spectra-pathological signatures.Clinical and translational medicine · 2025Review
- Deuterium-oxide-assisted stimulated Raman scattering microscopy: metabolic imaging for precision medicine.Biophotonics discovery · 2025Review
- Photoactivatable Alkyne Tag for Photolabeling Biomolecules in Living Cells.Chembiochem : a European journal of chemical biology · 2025Article
- Time-deterministic cryo-optical microscopy.Light, science & applications · 2025Article
- Two-dimensional bond-selective fluorescence spectroscopy: violations of the resonance condition, vibrational cooling rate dispersion, and super-multiplex imaging.Chemical science · 2025Article
- Quantum theory of stimulated Raman scattering microscopy.Chemical physics reviews · 2025Review
- Emerging Approaches for Studying Lipid Dynamics, Metabolism, and Interactions in Cells.Annual review of biochemistry · 2025Review
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Raman imaging as a window into cellular complexity: a future perspective.Nature methods · 2025Article
- Principle of Stimulated Raman Scattering Microscopy: Emerging at High Spatiotemporal Limits.The journal of physical chemistry. C, Nanomaterials and interfaces · 2025Article
- Towards high-accuracy bacterial taxonomy identification using phenotypic single-cell Raman spectroscopy data.ISME communications · 2025Article
- Raman Imaging of Targeted Drug Delivery with DNA-Based Nano-Optical Devices.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Single-cell multiparameter measurement has been increasingly recognized as a key technology toward systematic understandings of complex molecular and cellular functions in biological systems. Despite extensive efforts in analytical techniques, it is still generally challenging for existing methods to decipher a large number of phenotypes in a single living cell. Herein we devise a multiplexed Raman probe panel with sharp and mutually resolvable Raman peaks to simultaneously quantify cell surface proteins, endocytosis activities, and metabolic dynamics of an individual live cell. When coupling it to whole-cell spontaneous Raman micro-spectroscopy, we demonstrate the utility of this technique in 14-plexed live-cell profiling and phenotyping under various drug perturbations. In particular, single-cell multiparameter measurement enables powerful clustering, correlation, and network analysis with biological insights. This profiling platform is compatible with live-cell cytometry, of low instrument complexity and capable of highly multiplexed measurement in a robust and straightforward manner, thereby contributing a valuable tool for both basic single-cell biology and translation applications such as high-content cell sorting and drug discovery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.