Evidence map›Paper›PMID 34099545›Full record

ArticleJournal of immunology (Baltimore, Md. : 1950)2021

Defining T Cell Subsets in Human Tonsils Using ChipCytometry.

Joachim P Hagel, Kyle Bennett, Francesca Buffa, Paul Klenerman, Christian B Willberg, Kate Powell

Open access · hybridAbstract readComment
In one paragraph

Article in Journal of immunology (Baltimore, Md. : 1950), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
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  4. Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Joachim P HagelPeter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom; paul.klenerman@medawar.ox.ac.uk joachim.hagel@ndm.ox.ac.uk.ORCID 0000-0002-9952-1812
Kyle BennettPeter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.ORCID 0000-0003-0220-9097
Francesca BuffaComputational Biology and Integrative Genomics, Department of Oncology, University of Oxford, Oxford, United Kingdom.ORCID 0000-0003-0409-406X
Paul KlenermanPeter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom; paul.klenerman@medawar.ox.ac.uk joachim.hagel@ndm.ox.ac.uk.ORCID 0000-0003-4307-9161
Christian B WillbergPeter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.ORCID 0000-0001-5299-9344
Kate PowellPeter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.ORCID 0000-0002-1826-953X
University of Oxford · GBJohn Radcliffe Hospital · GBNuffield Orthopaedic Centre · GB

Funding

Cancer Research UK 23969Wellcome Trust 109965Wellcome Trust 109965/Z/15/Z
6 · The paper itself

Abstract

ChipCytometry is a multiplex imaging method that can be used to analyze either cell suspensions or tissue sections. Images are acquired by iterative cycles of immunostaining with fluorescently labeled Abs, followed by photobleaching, which allows the accumulation of multiple markers on a single sample. In this study, we explored the feasibility of using ChipCytometry to identify and phenotype cell subsets, including rare cell types, using a combination of tissue sections and single-cell suspensions. Using ChipCytometry of tissue sections, we successfully demonstrated the architecture of human palatine tonsils, including the B and T cell zones, and characterized subcompartments such as the B cell mantle and germinal center zone, as well as intrafollicular PD1-expressing CD4

Indexed as

Germinal CenterPalatine TonsilB-LymphocytesHumansT-Lymphocytes, Helper-InducerT-Lymphocyte Subsets

Identifiers

PMID34099545
PMCPMC8278278
OpenAlexW3168930323

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.