Evidence map›Paper›PMID 34099175›Full record

ArticleMethods in enzymology2021

VoltageFluor dyes and fluorescence lifetime imaging for optical measurement of membrane potential.

Anneliese M M Gest, Susanna K Yaeger-Weiss, Julia R Lazzari-Dean, Evan W Miller

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Anneliese M M GestDepartment of Chemistry, University of California, Berkeley, CA, United States.
Susanna K Yaeger-WeissDepartment of Chemistry, University of California, Berkeley, CA, United States.
Julia R Lazzari-DeanDepartment of Chemistry, University of California, Berkeley, CA, United States.
Evan W MillerDepartment of Chemistry, University of California, Berkeley, CA, United States; Department of Molecular & Cell Biology, University of California, Berkeley, CA, United States; Helen Wills Neuroscience Institute, University of California, Berkeley, CA, United States. Electronic address: evanwmiller@berkeley.edu.
University of California, Berkeley · US

Funding

MOLECULAR BIOPHYSICS TRAINING GRANTT32GM008295 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI HURLEY, JAMES H, WEMMER, DAVID E · 1989 to 2022
$8.1M
New Chemical Tools for Exploring Cellular PhysiologyR35GM119855 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI MILLER, EVAN WALKER · 2016 to 2020
$1.3M
NIGMS NIH HHS R35 GM119855NIGMS NIH HHS T32 GM008295
6 · The paper itself

Abstract

Membrane potential is a fundamental biophysical parameter common to all of cellular life. Traditional methods to measure membrane potential rely on electrodes, which are invasive and low-throughput. Optical methods to measure membrane potential are attractive because they have the potential to be less invasive and higher throughput than classic electrode based techniques. However, most optical measurements rely on changes in fluorescence intensity to detect changes in membrane potential. In this chapter, we discuss the use of fluorescence lifetime imaging microscopy (FLIM) and voltage-sensitive fluorophores (VoltageFluors, or VF dyes) to estimate the millivolt value of membrane potentials in living cells. We discuss theory, application, protocols, and shortcomings of this approach.

Indexed as

Fluorescent DyesOptical ImagingMembrane PotentialsMicroscopy, FluorescenceFluorescent DyesElectrophysiologyFLIMFluorescence lifetimeImagingMembrane potentialMicroscopyVoltage-sensitive fluorophore

Identifiers

PMID34099175
PMCPMC8356362
OpenAlexW3150865989

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.