Evidence map›Paper›PMID 34097383›Full record

ArticleACS synthetic biology2021

Construction of DNA Tools for Hyperexpression in

Eftychios Frangedakis, Fernando Guzman-Chavez, Marius Rebmann, Kasey Markel, Ying Yu, Artemis Perraki, Sze Wai Tse, Yang Liu, Jenna Rever, Susanna Sauret-Gueto and 3 more

Open access · hybridAbstract read
In one paragraph

Article in ACS synthetic biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Article
  3. Laser ablation microscopy reveals apical notch, apical dominance, and meristem regeneration dynamics inProceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. PhytoKeys · 2024
    Article
  11. The complete chloroplast genome of an Antarctic moss,Mitochondrial DNA. Part B, Resources · 2024
    Article
  12. An enhancer trap system to track developmental dynamics in Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2023
    Article
  13. What can hornworts teach us?Frontiers in plant science · 2023
    Review
  14. The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2023
    Article
  15. Article
  16. Review
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 3 countries.

Eftychios FrangedakisDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.ORCID 0000-0002-3483-8464
Fernando Guzman-ChavezDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.
Marius RebmannDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.ORCID 0000-0003-1462-0975
Kasey MarkelDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.ORCID 0000-0002-8285-3888
Ying YuCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Artemis PerrakiDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.
Sze Wai TseDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.
Yang LiuFairy Lake Botanical Garden & Chinese Academy of Sciences, Shenzhen, Guangdong 518004, China.
Jenna ReverDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.
Susanna Sauret-GuetoDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.ORCID 0000-0003-3792-8278
Bernard GoffinetDepartment of Ecology and Evolutionary Biology, University of Connecticut, Storrs, Connecticut 06269-3043, United States.
Harald SchneiderCenter for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Yunnan 666303, China.
Jim HaseloffDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.ORCID 0000-0003-4793-8058
University of Cambridge · GBChinese Academy of Sciences · CNHangzhou Normal University · CNUniversity of Connecticut · USXishuangbanna Tropical Botanical Garden · CN

Funding

Biotechnology and Biological Sciences Research Council BB/F011458/1Biotechnology and Biological Sciences Research Council BB/L014130/1
6 · The paper itself

Abstract

Chloroplasts are attractive platforms for synthetic biology applications since they are capable of driving very high levels of transgene expression, if mRNA production and stability are properly regulated. However, plastid transformation is a slow process and currently limited to a few plant species. The liverwort

Indexed as

ChloroplastsDNA, RecombinantGenes, PlantMarchantiaPlant ProteinsProtein BindingSynthetic BiologyTranscription, GeneticTranscriptomeTransformation, GeneticDNA, RecombinantPlant Proteinschloroplastgene assemblyMarchantiaplantplastometranscriptome

Identifiers

PMID34097383
PMCPMC8296666
OpenAlexW3171024159

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.