ArticleMethods in molecular biology (Clifton, N.J.)2021
Determination of Metal Content in Drosophila melanogaster During Metal Exposure.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed, 4 citations in OpenAlex.
- Divalent metal content in diet affects severity of manganese toxicity in Drosophila.Biology open · 2024Article
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Abstract
Trace metal elements, such as zinc, iron, copper, and manganese, play catalytic or structural roles in many enzymes and numerous proteins, and accordingly, contribute to a variety of fundamental biological processes. During the past decade, the fruit fly (Drosophila melanogaster) has become an important model organism for elucidating metal homeostasis in metazoan. We have been using Drosophila as a model to study metal metabolism for many years and have optimized simple and robust assays for determining the metal content in Drosophila, such as inductively coupled plasma mass spectrometry (ICP-MS), the activity assay of enzymes dependent on metals, and staining metal ions in tissues of Drosophila. In this chapter, we present the step-by-step detailed methods for detecting the metal content in Drosophila melanogaster during metal toxicity study.
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